| Literature DB >> 17996372 |
Maria Emília Bodini Santiago1, Rosemeri O Vasconcelos, Karina Reinaldo Fattori, Danísio Prado Munari, Aparecida de Fátima Michelin, Valéria Marçal Felix Lima.
Abstract
Blood and bone marrow samples were taken from 112 Didelphis spp., collected between March 2005 and February 2006, from urban and peri-urban areas of Bauru, São Paulo State, Brazil, to evaluate the hypothesis that these animals might constitute a reservoir of Leishmania spp. Anti-Leishmania ssp. antibodies were screened in the serum samples using an enzyme-linked immuno-sorbent assay (ELISA) and the polymerase chain reaction (PCR). PCR was performed on fragments of DNA samples from Leishmania spp. using primers 13A and 13B, and showed a positive outcome in 91.6% of the 112 samples tested. Of the 107 samples analyzed by ELISA, 71% were positive. Evidence of epidemiological risk factors such as a circulating parasite and freely moving vectors suggests that Didelphis spp. may participate in the transmission cycle of Leishmania spp. in Bauru.Entities:
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Year: 2007 PMID： 17996372 DOI： 10.1016/j.vetpar.2007.09.026
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738