OBJECTIVES: To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort. DESIGN AND METHODS: The RT-PCR assay for PCA3 is based on target-specific lanthanide probes. Peripheral blood from 91 prostatic cancer/disorder patients and healthy controls was assayed for PCA3 and prostate-specific antigen (PSA) expression. RESULTS: The dynamic range of the assay reaches over eight orders of magnitude and the limit of quantification is 800 copies per milliliter blood. Peripheral blood from 2/9 patients with metastasized cancers were PCA3 positive, whereas all the other samples were negative. Eight samples were PSA positive. CONCLUSIONS: The degree of PCA3 positivity in circulating cells from prostate cancer patients is low compared to that of PSA. In contrast to some previous reports, we found no PCA3 expression in healthy individuals.
OBJECTIVES: To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort. DESIGN AND METHODS: The RT-PCR assay for PCA3 is based on target-specific lanthanide probes. Peripheral blood from 91 prostatic cancer/disorderpatients and healthy controls was assayed for PCA3 and prostate-specific antigen (PSA) expression. RESULTS: The dynamic range of the assay reaches over eight orders of magnitude and the limit of quantification is 800 copies per milliliter blood. Peripheral blood from 2/9 patients with metastasized cancers were PCA3 positive, whereas all the other samples were negative. Eight samples were PSA positive. CONCLUSIONS: The degree of PCA3 positivity in circulating cells from prostate cancerpatients is low compared to that of PSA. In contrast to some previous reports, we found no PCA3 expression in healthy individuals.
Authors: Pauliina Helo; Angel M Cronin; Daniel C Danila; Sven Wenske; Rita Gonzalez-Espinoza; Aseem Anand; Michael Koscuiszka; Riina-Minna Väänänen; Kim Pettersson; Felix K-H Chun; Thomas Steuber; Hartwig Huland; Bertrand D Guillonneau; James A Eastham; Peter T Scardino; Martin Fleisher; Howard I Scher; Hans Lilja Journal: Clin Chem Date: 2009-02-20 Impact factor: 8.327
Authors: Riina-Minna Väänänen; Hans Lilja; Leni Kauko; Pauliina Helo; Henna Kekki; Angel M Cronin; Andrew J Vickers; Martti Nurmi; Kalle Alanen; Anders Bjartell; Kim Pettersson Journal: Urology Date: 2014-02 Impact factor: 2.649
Authors: Karina Marangoni; Adriana F Neves; Rafael M Rocha; Paulo R Faria; Patrícia T Alves; Aline G Souza; Patrícia T Fujimura; Fabiana A A Santos; Thaise G Araújo; Laura S Ward; Luiz R Goulart Journal: Sci Rep Date: 2015-07-15 Impact factor: 4.379