Alignment of genomic sequences using DIALIGN.

DIALIGN is a software program for multiple alignment of DNA or protein sequences that combines global and local alignment features. During the last years, the program has been used extensively to compare syntenic regions in genomic sequences. An anchoring option speeds up the alignment procedure and makes it possible to use user-defined constraints to improve the quality of the program output. This chapter explains features of DIALIGN that are useful if genomic sequences are to be aligned. The program is online available through Göttingen Bioinformatics Compute Server at http://dialign.gobics.de/.