| Literature DB >> 17993673 |
Abstract
PSI-BLAST (Position-Specific Iterative Basic Local Alignment Search Tool) derives a position-specific scoring matrix (PSSM) or profile from the multiple sequence alignment of sequences detected above a given score threshold using protein-protein BLAST. This PSSM is used to further search the database for new matches, and is updated for subsequent iterations with these newly detected sequences. Thus, PSI-BLAST provides a means of detecting distant relationships between proteins. In this chapter, we discuss practical aspects of using PSI-BLAST and provide a tutorial on how to uncover distant relationships between proteins and use them to reach biologically meaningful conclusions.Mesh：
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Year: 2007 PMID： 17993673 PMCID： PMC4781153 DOI： 10.1007/978-1-59745-514-5_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745