Li-bo Zhao1, You Jin, Jia-ling Wang. 1. Department of Pharmacology, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430030, China.
Abstract
OBJECTIVE: To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule. METHOD: A Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1). RESULT: The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol. CONCLUSION: The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.
OBJECTIVE: To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule. METHOD: A Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1). RESULT: The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol. CONCLUSION: The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.