| Literature DB >> 17990888 |
Daniel J Klein1, Michael D Been, Adrian R Ferré-D'Amaré.
Abstract
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.Entities:
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Year: 2007 PMID： 17990888 DOI： 10.1021/ja0768441
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419