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Literature DB >> 17986777

Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis.

Takeshi Fukuda¹, Danya Isogawa, Madoka Takagi, Michiko Kato-Murai, Hisashi Kimoto, Hideo Kusaoke, Mitsuyoshi Ueda, Shin-Ichiro Suye.

Abstract

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.

Entities: Chemical Species

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Year: 2007 PMID： 17986777 DOI： 10.1271/bbb.70315

Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043

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Cited

7 in total

1. Construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display.

Authors: Xiaobo Li; Xiaobao Jin; Xuemei Lu; Fujiang Chu; Juan Shen; Yan Ma; Manyu Liu; Jiayong Zhu
Journal: World J Microbiol Biotechnol Date: 2014-06-07 Impact factor: 3.312

2. A temperature-induced chitosanase bacterial cell-surface display system for the efficient production of chitooligosaccharides.

Authors: Qianqian Li; Tuantuan Wang; Yangzhi Ye; Shimin Guan; Baoguo Cai; Shuo Zhang; Shaofeng Rong
Journal: Biotechnol Lett Date: 2021-05-16 Impact factor: 2.461

3. Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts.

Authors: Hoang-Minh Nguyen; Geir Mathiesen; Elena Maria Stelzer; Mai Lan Pham; Katarzyna Kuczkowska; Alasdair Mackenzie; Jane W Agger; Vincent G H Eijsink; Montarop Yamabhai; Clemens K Peterbauer; Dietmar Haltrich; Thu-Ha Nguyen
Journal: Microb Cell Fact Date: 2016-10-04 Impact factor: 5.328

Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis.

1. Construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display.

2. A temperature-induced chitosanase bacterial cell-surface display system for the efficient production of chitooligosaccharides.

3. Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts.

4. Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum.

Review 5. Cell-surface engineering of yeasts for whole-cell biocatalysts.

6. Yeast cell factories for fine chemical and API production.

7. Arming Technology in Yeast-Novel Strategy for Whole-cell Biocatalyst and Protein Engineering.