| Literature DB >> 17984532 |
Kazuo Yamamoto1, Fumiko Yasukawa, Seiichiro Ito.
Abstract
A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N- and O-glycans were immobilized on multiplexed beads, and the specificities of several kinds of sugar chains were measured in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples, and allows the reliable elucidation of sugar-binding properties of lectins under identical conditions.Entities:
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Year: 2007 PMID： 17984532 DOI： 10.1007/978-1-59745-303-5_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745