BACKGROUND: Bronchodilator responses are a hallmark of asthma and a subset of chronic obstructive pulmonary disease (COPD). We evaluated subjects with COPD and computed tomographic (CT) scan evidence of emphysema to determine the biomarker profile associated with a bronchodilator response. OBJECTIVE: We investigated whether subjects with COPD and a bronchodilator response had increased levels of bronchoalveolar lavage (BAL) fluid eosinophil biomarkers, T(H)2 cytokines, CC chemokines, and serum allergen-specific IgE. METHODS: All patients with COPD and control subjects (n = 31) had chest CT scans to detect emphysema and subsequent pulmonary function studies, BAL for biomarkers, and serum IgE measurements. RESULTS: CT scan score, FEV(1), carbon monoxide single-breath diffusion capacity, and BAL fluid neutrophil biomarker levels were similar in subjects with COPD who had or did not have a bronchodilator response of greater than 12%. In contrast, levels of BAL fluid eosinophil biomarkers (eosinophil cationic protein [ECP] and eotaxin-1) were greater in patients with COPD with a bronchodilator response, whereas T(H)2 cytokines were not detectable in any patients with COPD. BAL fluid ECP and eotaxin-1 levels correlated with CT scan extent of emphysema. Immunostaining of COPD lung sections from a separate cohort of subjects with COPD and healthy subjects demonstrated epithelial expression of eotaxin-1 but no lung expression of IL-4 or IL-5. CONCLUSION: Subjects with COPD diagnosed on the basis of the presence of emphysema on CT scan who have a bronchodilator response have increased levels of BAL ECP and eotaxin-1 but not T(H)2 cytokines. CLINICAL IMPLICATIONS: Eosinophil biomarkers (ECP-1 and eotaxin-1) might identify a subset of subjects with COPD with emphysema on CT scans who have a bronchodilator response and an increased extent of emphysema on CT scanning.
BACKGROUND: Bronchodilator responses are a hallmark of asthma and a subset of chronic obstructive pulmonary disease (COPD). We evaluated subjects with COPD and computed tomographic (CT) scan evidence of emphysema to determine the biomarker profile associated with a bronchodilator response. OBJECTIVE: We investigated whether subjects with COPD and a bronchodilator response had increased levels of bronchoalveolar lavage (BAL) fluid eosinophil biomarkers, T(H)2 cytokines, CC chemokines, and serum allergen-specific IgE. METHODS: All patients with COPD and control subjects (n = 31) had chest CT scans to detect emphysema and subsequent pulmonary function studies, BAL for biomarkers, and serum IgE measurements. RESULTS: CT scan score, FEV(1), carbon monoxide single-breath diffusion capacity, and BAL fluid neutrophil biomarker levels were similar in subjects with COPD who had or did not have a bronchodilator response of greater than 12%. In contrast, levels of BAL fluid eosinophil biomarkers (eosinophil cationic protein [ECP] and eotaxin-1) were greater in patients with COPD with a bronchodilator response, whereas T(H)2 cytokines were not detectable in any patients with COPD. BAL fluid ECP and eotaxin-1 levels correlated with CT scan extent of emphysema. Immunostaining of COPD lung sections from a separate cohort of subjects with COPD and healthy subjects demonstrated epithelial expression of eotaxin-1 but no lung expression of IL-4 or IL-5. CONCLUSION: Subjects with COPD diagnosed on the basis of the presence of emphysema on CT scan who have a bronchodilator response have increased levels of BAL ECP and eotaxin-1 but not T(H)2 cytokines. CLINICAL IMPLICATIONS: Eosinophil biomarkers (ECP-1 and eotaxin-1) might identify a subset of subjects with COPD with emphysema on CT scans who have a bronchodilator response and an increased extent of emphysema on CT scanning.
Authors: M Bafadhel; M McCormick; S Saha; S McKenna; M Shelley; B Hargadon; V Mistry; C Reid; D Parker; P Dodson; M Jenkins; A Lloyd; P Rugman; Paul Newbold; C E Brightling Journal: Respiration Date: 2011-09-07 Impact factor: 3.580
Authors: Kenneth I Berger; Deepak R Pradhan; Roberta M Goldring; Beno W Oppenheimer; William N Rom; Leopoldo N Segal Journal: ERJ Open Res Date: 2016-11-29
Authors: Jeanine M D'Armiento; Steven M Scharf; Michael D Roth; John E Connett; Andrew Ghio; David Sternberg; Jonathan G Goldin; Thomas A Louis; Jenny T Mao; George T O'Connor; Joe W Ramsdell; Andrew L Ries; Neil W Schluger; Frank C Sciurba; Melissa A Skeans; Helen Voelker; Robert E Walter; Christine H Wendt; Gail G Weinmann; Robert A Wise; Robert F Foronjy Journal: Respir Res Date: 2009-11-19
Authors: Brendan J Carolan; Grant Hughes; Jarrett Morrow; Craig P Hersh; Wanda K O'Neal; Stephen Rennard; Sreekumar G Pillai; Paula Belloni; Debra A Cockayne; Alejandro P Comellas; Meilan Han; Rachel L Zemans; Katerina Kechris; Russell P Bowler Journal: Respir Res Date: 2014-10-12