Bu-Fang Ren1, Lian-Fu Deng, Jun Wang, Ya-Ping Zhu, Li Wei, Qi Zhou. 1. Shanghai Institute of Traumatology & Orthopaedics and Department of Orthopaedics, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200025, China.
Abstract
OBJECTIVES: Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism. RESULTS: Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1alpha (HIF-1alpha) protein level. On the contrary, deletion of HIF-1alpha led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3. CONCLUSION: These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1alpha.
OBJECTIVES: Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism. RESULTS: Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1alpha (HIF-1alpha) protein level. On the contrary, deletion of HIF-1alpha led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3. CONCLUSION: These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1alpha.
Authors: Carolina Balmaceda-Aguilera; Christian Cortés-Campos; Manuel Cifuentes; Bruno Peruzzo; Lauren Mack; Juan Carlos Tapia; Karina Oyarce; María Angeles García; Francisco Nualart Journal: PLoS One Date: 2012-02-28 Impact factor: 3.240