Literature DB >> 1798342

High sensitivity HPLC assay for GABA in brain dialysis studies.

A Bourdelais1, P W Kalivas.   

Abstract

The measurement of GABA in brain dialysis experiments involves precolumn derivatization of the amino acid with o-phthaldehyde (OPA) in the presence of a thiol and subsequent high-pressure liquid chromatography with electrochemical detection. A method is described which employs an internal standard and coulometric preoxidation (+ 0.2 V) to eliminate unconjugated OPA/thiol prior to final oxidation (+ 0.4 V) of the derivatized sample. This allows for more efficient separation, providing a retention time for GABA of 9-10 min, a detection sensitivity of 2 to 5 x 10(-14) mol/sample and chromatographic stability for at least 2 weeks of daily use.

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Year:  1991        PMID： 1798342     DOI： 10.1016/0165-0270(91)90077-d

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  5 in total

1.  High sensitivity HPLC method for analysis of in vivo extracellular GABA using optimized fluorescence parameters for o-phthalaldehyde (OPA)/sulfite derivatives.

Authors:  Shannon L Zandy; James M Doherty; Nathan D Wibisono; Rueben A Gonzales
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2017-04-03       Impact factor: 3.205

2.  gamma-Aminobutyric acid is an inhibitory neurotransmitter restricting the release of luteinizing hormone-releasing hormone before the onset of puberty.

Authors:  D Mitsushima; D L Hei; E Terasawa
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-04       Impact factor: 11.205

3.  Group II metabotropic glutamate receptor type 2 allosteric potentiators prevent sodium lactate-induced panic-like response in panic-vulnerable rats.

Authors:  Philip L Johnson; Stephanie D Fitz; Eric A Engleman; Kjell A Svensson; Jeffrey M Schkeryantz; Anantha Shekhar
Journal:  J Psychopharmacol       Date:  2012-08-21       Impact factor: 4.153

4.  Continuous cocaine induces persisting alterations in dopamine overflow in caudate following perfusion with a D1 agonist.

Authors:  A Keys; G Ellison
Journal:  J Neural Transm Gen Sect       Date:  1994

Review 5.  Role of the endogenous cannabinoid system in nicotine addiction: novel insights.

Authors:  Islam Hany Gamaleddin; Jose M Trigo; Aliou B Gueye; Alexander Zvonok; Alexandros Makriyannis; Steven R Goldberg; Bernard Le Foll
Journal:  Front Psychiatry       Date:  2015-03-25       Impact factor: 4.157
  5 in total

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