Literature DB >> 17981875

A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters.

Akira Nozawa1, Hideaki Nanamiya, Takuji Miyata, Nicole Linka, Yaeta Endo, Andreas P M Weber, Yuzuru Tozawa.   

Abstract

We describe here a novel proteoliposome reconstitution system for functional analysis of plant membrane transporters that is based on a modified wheat germ cell-free translation system. We established optimized conditions for the reconstitution system with Arabidopsis thaliana phosphoenolpyruvate/phosphate translocator 1 (AtPPT1) as a model transporter. A high activity of AtPPT1 was achieved by synthesis of the protein in the presence of both a detergent such as Brij35 and liposomes. We also determined the substrate specificities of three putative rice PPT homologs with this system. The cell-free proteoliposome reconstitution system provides a valuable tool for functional analysis of transporter proteins.

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Year:  2007        PMID： 17981875     DOI： 10.1093/pcp/pcm150

Source DB:  PubMed          Journal:  Plant Cell Physiol        ISSN： 0032-0781            Impact factor:   4.927


  20 in total

1.  Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.

Authors:  Yi Ma; Daniela Münch; Tanja Schneider; Hans-Georg Sahl; Ahmed Bouhss; Umesh Ghoshdastider; Jufang Wang; Volker Dötsch; Xiaoning Wang; Frank Bernhard
Journal:  J Biol Chem       Date:  2011-09-20       Impact factor: 5.157

Review 2.  C4 cycles: past, present, and future research on C4 photosynthesis.

Authors:  Jane A Langdale
Journal:  Plant Cell       Date:  2011-11-29       Impact factor: 11.277

3.  Cell-free expression and stable isotope labelling strategies for membrane proteins.

Authors:  Solmaz Sobhanifar; Sina Reckel; Friederike Junge; Daniel Schwarz; Lei Kai; Mikhail Karbyshev; Frank Löhr; Frank Bernhard; Volker Dötsch
Journal:  J Biomol NMR       Date:  2009-08-13       Impact factor: 2.835

4.  Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipid.

Authors:  Kazumi Shimono; Mie Goto; Takashi Kikukawa; Seiji Miyauchi; Mikako Shirouzu; Naoki Kamo; Shigeyuki Yokoyama
Journal:  Protein Sci       Date:  2009-10       Impact factor: 6.725

5.  Practical cell-free protein synthesis system using purified wheat embryos.

Authors:  Kazuyuki Takai; Tatsuya Sawasaki; Yaeta Endo
Journal:  Nat Protoc       Date:  2010-01-21       Impact factor: 13.491

6.  An Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography.

Authors:  Tuan A Nguyen; Samantha S Lieu; Geoffrey Chang
Journal:  J Mol Microbiol Biotechnol       Date:  2010-02-17

7.  Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.

Authors:  Michael A Goren; Brian G Fox
Journal:  Protein Expr Purif       Date:  2008-08-15       Impact factor: 1.650

Review 8.  Cell-free translation of integral membrane proteins into unilamelar liposomes.

Authors:  Michael A Goren; Akira Nozawa; Shin-ichi Makino; Russell L Wrobel; Brian G Fox
Journal:  Methods Enzymol       Date:  2009       Impact factor: 1.600

9.  The Arabidopsis Plastidial Glucose-6-Phosphate Transporter GPT1 is Dually Targeted to Peroxisomes via the Endoplasmic Reticulum.

Authors:  Marie-Christin Baune; Hannes Lansing; Kerstin Fischer; Tanja Meyer; Lennart Charton; Nicole Linka; Antje von Schaewen
Journal:  Plant Cell       Date:  2020-02-28       Impact factor: 11.277

10.  The cell-free integration of a polytopic mitochondrial membrane protein into liposomes occurs cotranslationally and in a lipid-dependent manner.

Authors:  Ashley R Long; Catherine C O'Brien; Nathan N Alder
Journal:  PLoS One       Date:  2012-09-25       Impact factor: 3.240
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