BACKGROUND: An increase of leaky vasculature is vital for the growth and metastasis of hepatocellular carcinoma (HCC). The paracellular permeability-regulating proteins in tumor vessels and adjacent sinusoids have not been studied in HCC patients. METHODS: Expression of an endothelial tight junction protein claudin-5 (CL-5) and a standard endothelial marker CD34 were immunohistochemically examined in resected specimens from 51 HCC cases. The relationship between hepatitic or fibrotic grade and CL-5 expression pattern in sinusoidal endothelial cells (SECs) was evaluated in the tumor-adjacent tissues. Microvessel density (MVD) highlighted by CD34 or CL-5 was examined in tumor tissues. RESULTS: In the normal liver, a ubiquitous CL-5 expression was seen in SECs, the arteries, and portal veins but not in the central veins. Sinusoidal CL-5 expression was down-regulated according to the increase of hepatitic or fibrotic grade. Poor differentiation and vasculobiliary invasion were significantly associated with a lower CL-5-MVD but not CD34-MVD. By multivariate analysis, vasculobiliary invasion and lower CL-5-MVD were independent factors associated with a lower postoperative overall survival rate. CONCLUSIONS: Attenuated CL-5 expression in SECs may be related to SEC dysfunction in injured liver. Down-regulated CL-5 expression in tumor vessels may serve as a potential marker for poor prognosis in HCC.
BACKGROUND: An increase of leaky vasculature is vital for the growth and metastasis of hepatocellular carcinoma (HCC). The paracellular permeability-regulating proteins in tumor vessels and adjacent sinusoids have not been studied in HCC patients. METHODS: Expression of an endothelial tight junction protein claudin-5 (CL-5) and a standard endothelial marker CD34 were immunohistochemically examined in resected specimens from 51 HCC cases. The relationship between hepatitic or fibrotic grade and CL-5 expression pattern in sinusoidal endothelial cells (SECs) was evaluated in the tumor-adjacent tissues. Microvessel density (MVD) highlighted by CD34 or CL-5 was examined in tumor tissues. RESULTS: In the normal liver, a ubiquitous CL-5 expression was seen in SECs, the arteries, and portal veins but not in the central veins. Sinusoidal CL-5 expression was down-regulated according to the increase of hepatitic or fibrotic grade. Poor differentiation and vasculobiliary invasion were significantly associated with a lower CL-5-MVD but not CD34-MVD. By multivariate analysis, vasculobiliary invasion and lower CL-5-MVD were independent factors associated with a lower postoperative overall survival rate. CONCLUSIONS: Attenuated CL-5 expression in SECs may be related to SEC dysfunction in injured liver. Down-regulated CL-5 expression in tumor vessels may serve as a potential marker for poor prognosis in HCC.
Authors: Aron Somorácz; Anna Korompay; Péter Törzsök; Attila Patonai; Boglárka Erdélyi-Belle; Gábor Lotz; Zsuzsa Schaff; András Kiss Journal: Pathol Oncol Res Date: 2014-03-21 Impact factor: 3.201
Authors: Cyrill Géraud; Konstantin Evdokimov; Beate K Straub; Wiebke K Peitsch; Alexandra Demory; Yvette Dörflinger; Kai Schledzewski; Astrid Schmieder; Peter Schemmer; Hellmut G Augustin; Peter Schirmacher; Sergij Goerdt Journal: PLoS One Date: 2012-04-03 Impact factor: 3.240
Authors: Bu-Yeo Kim; Dong Wook Choi; Seon Rang Woo; Eun-Ran Park; Je-Geun Lee; Su-Hyeon Kim; Imhoi Koo; Sun-Hoo Park; Chul Ju Han; Sang Bum Kim; Young Il Yeom; Suk-Jin Yang; Ami Yu; Jae Won Lee; Ja June Jang; Myung-Haing Cho; Won Kyung Jeon; Young Nyun Park; Kyung-Suk Suh; Kee-Ho Lee Journal: BMC Genomics Date: 2015-04-10 Impact factor: 3.969