DNA-damaging, mutagenic, clastogenic and cell-cell communication inhibitory properties of gamma-oryzanol.

As a part of short-term safety assessment of gamma-Oryzanol, the genotoxic or the carcinogenic initiation activity was studied in three genetic toxicity tests and the promotion activity was studied in a cell-cell communication inhibitory test. gamma-Oryzanol showed the negative response in the bacterial DNA repair test (Rec-assay), the bacterial reverse mutation tests (Ames test) and the rat bone marrow chromosome aberration test. Also, gamma-Oryzanol showed the negative response in the metabolic cooperation inhibition test using Chinese hamster V79 cells.