Literature DB >> 17978341

Detection of "apoptosis-like" changes during the cryopreservation process in equine sperm.

C Ortega-Ferrusola1, Y Sotillo-Galán, E Varela-Fernández, J M Gallardo-Bolaños, A Muriel, L González-Fernández, J A Tapia, F J Peña.   

Abstract

The kinematics of the appearance of apoptotic markers was studied by flow cytometry and immunoblot assays in equine spermatozoa subjected to freezing and thawing. Caspase activity, low mitochondrial membrane potential, and increases in sperm membrane permeability were observed in all of the phases of the cryopreservation procedure. Freezing and thawing caused an increase in membrane permeability and changes in the pattern of caspase activity; decreases in mitochondrial membrane potential were observed after centrifugation and cooling to 4 degrees C and after freezing and thawing. It is proposed that sperm mitochondria may be directly involved in the subtle damage that is present in most spermatozoa surviving freezing and thawing.

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Year:  2007        PMID： 17978341     DOI： 10.2164/jandrol.107.003640

Source DB:  PubMed          Journal:  J Androl        ISSN： 0196-3635


  26 in total

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10.  Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation.

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