Literature DB >> 1797805

Materials for enhancing cell adhesion by immobilization of cell-adhesive peptide.

Y Ito1, M Kajihara, Y Imanishi.   

Abstract

Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glow-discharged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodiimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film.

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Year:  1991        PMID： 1797805     DOI： 10.1002/jbm.820251102

Source DB:  PubMed          Journal:  J Biomed Mater Res        ISSN： 0021-9304


  10 in total

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Authors:  Jiehua Li; Mingming Ding; Qiang Fu; Hong Tan; Xingyi Xie; Yinping Zhong
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10.  A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.

Authors:  Sha Jin; Huantong Yao; Jennifer L Weber; Zara K Melkoumian; Kaiming Ye
Journal:  PLoS One       Date:  2012-11-30       Impact factor: 3.240
  10 in total

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