Literature DB >> 17977534

Kinetic characterization of mammalian ceramide synthases: determination of K(m) values towards sphinganine.

Sujoy Lahiri1, Hyunmi Lee, Judith Mesicek, Zvi Fuks, Adriana Haimovitz-Friedman, Richard N Kolesnick, Anthony H Futerman.   

Abstract

Ceramide is a key metabolite in the pathway of sphingolipid biosynthesis. In mammals, ceramide is synthesized by N-acylation of a sphingoid long-chain base by a family of ceramide synthases (CerS), each of which displays a high specificity towards acyl CoAs of different chain lengths. We now optimize a previously-described assay for measuring CerS activity for use upon over-expression of mammalian CerS, and using these conditions, establish the K(m) value of each CerS towards sphinganine. Remarkably, the K(m) values towards sphinganine are all similar, ranging from 2 to 5microM, even for CerS proteins that are able to use more than one acyl CoA for ceramide synthesis (i.e. CerS4). The availability of this assay will permit further accurate characterization of the kinetic parameters of mammalian CerS proteins.

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Year:  2007        PMID： 17977534     DOI： 10.1016/j.febslet.2007.10.018

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  40 in total

1.  A fluorescent assay for ceramide synthase activity.

Authors:  Hyun Joon Kim; Qiao Qiao; Hamish D Toop; Jonathan C Morris; Anthony S Don
Journal:  J Lipid Res       Date:  2012-06-01       Impact factor: 5.922

2.  A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction.

Authors:  Rotem Tidhar; Kacee Sims; Eden Rosenfeld-Gur; Walter Shaw; Anthony H Futerman
Journal:  J Lipid Res       Date:  2014-11-03       Impact factor: 5.922

Review 3.  Sphingolipid and glycosphingolipid metabolic pathways in the era of sphingolipidomics.

Authors:  Alfred H Merrill
Journal:  Chem Rev       Date:  2011-09-26       Impact factor: 60.622

4.  Expression of Ceramide Synthase 6 Transcriptionally Activates Acid Ceramidase in a c-Jun N-terminal Kinase (JNK)-dependent Manner.

Authors:  Tejas S Tirodkar; Ping Lu; Aiping Bai; Matthew J Scheffel; Salih Gencer; Elizabeth Garrett-Mayer; Alicja Bielawska; Besim Ogretmen; Christina Voelkel-Johnson
Journal:  J Biol Chem       Date:  2015-04-03       Impact factor: 5.157

Review 5.  Ceramide-rich platforms in transmembrane signaling.

Authors:  Branka Stancevic; Richard Kolesnick
Journal:  FEBS Lett       Date:  2010-02-20       Impact factor: 4.124

6.  Modulation of ceramide synthase activity via dimerization.

Authors:  Elad L Laviad; Samuel Kelly; Alfred H Merrill; Anthony H Futerman
Journal:  J Biol Chem       Date:  2012-04-26       Impact factor: 5.157

7.  Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.

Authors:  Sergei A Novgorodov; Bill X Wu; Tatyana I Gudz; Jacek Bielawski; Tatiana V Ovchinnikova; Yusuf A Hannun; Lina M Obeid
Journal:  J Biol Chem       Date:  2011-05-25       Impact factor: 5.157

8.  Following the flux of long-chain bases through the sphingolipid pathway in vivo using mass spectrometry.

Authors:  Fernando Martínez-Montañés; Roger Schneiter
Journal:  J Lipid Res       Date:  2016-03-14       Impact factor: 5.922

9.  Ceramide synthesis is modulated by the sphingosine analog FTY720 via a mixture of uncompetitive and noncompetitive inhibition in an Acyl-CoA chain length-dependent manner.

Authors:  Sujoy Lahiri; Hyejung Park; Elad L Laviad; Xuequan Lu; Robert Bittman; Anthony H Futerman
Journal:  J Biol Chem       Date:  2009-04-08       Impact factor: 5.157

10.  Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies.

Authors:  Hyejung Park; Christopher A Haynes; Alison V Nairn; Michael Kulik; Stephen Dalton; Kelley Moremen; Alfred H Merrill
Journal:  J Lipid Res       Date:  2009-09-28       Impact factor: 5.922
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