| Literature DB >> 17976380 |
Sven Seibeck1, Berthold Borucki, Harald Otto, Katsuhiko Inomata, Htoi Khawn, Hideki Kinoshita, Norbert Michael, Tilman Lamparter, Maarten P Heyn.
Abstract
The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but the following millisecond-transition to Meta-R(C) is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-R(A)-like intermediate, since the subsequent syn to anti rotation around the C(5)-C(6) bond is prevented by the lock. The Meta-R(A)-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.Entities:
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Year: 2007 PMID： 17976380 DOI： 10.1016/j.febslet.2007.10.043
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124