BACKGROUND: Resveratrol (RV), a naturally occurring phytoalexin, exerts manifold biological effects against a variety of human tumor cell lines. In this study, the cytotoxic and biological effects of novel RV derivatives were investigated in prostate cancer cells. MATERIALS AND METHODS: Cytotoxicity of the compounds was assessed by clonogenic assays in PC-3, LNCaP and DU-145 human prostate cancer cell lines. Induction of apoptosis was studied by Hoechst-propidium-iodide double staining. Cell cycle phase distribution of prostate cancer cells was analyzed using flow cytometry. RESULTS: Methoxy- and hydroxy-substituted RV derivatives exerted cytotoxic effects against all three cell lines. The most potent compounds, 3,3',4,4',5,5'-hexahydroxy-stilbene and 3,4,4',5-tetramethoxystilbene, induced apoptosis at concentrations lower than RV and caused cell cycle arrest in the cell lines investigated. CONCLUSION: Introducing additional hydroxy- and methoxymoieties to the stilbene ring of RV is capable of enhancing its cytotoxic and pro-apoptotic effects in hormone-responsive and non-responsive prostate cancer cells.
BACKGROUND:Resveratrol (RV), a naturally occurring phytoalexin, exerts manifold biological effects against a variety of humantumor cell lines. In this study, the cytotoxic and biological effects of novel RV derivatives were investigated in prostate cancer cells. MATERIALS AND METHODS:Cytotoxicity of the compounds was assessed by clonogenic assays in PC-3, LNCaP and DU-145 humanprostate cancer cell lines. Induction of apoptosis was studied by Hoechst-propidium-iodide double staining. Cell cycle phase distribution of prostate cancer cells was analyzed using flow cytometry. RESULTS:Methoxy- and hydroxy-substituted RV derivatives exerted cytotoxic effects against all three cell lines. The most potent compounds, 3,3',4,4',5,5'-hexahydroxy-stilbene and 3,4,4',5-tetramethoxystilbene, induced apoptosis at concentrations lower than RV and caused cell cycle arrest in the cell lines investigated. CONCLUSION: Introducing additional hydroxy- and methoxymoieties to the stilbene ring of RV is capable of enhancing its cytotoxic and pro-apoptotic effects in hormone-responsive and non-responsive prostate cancer cells.
Authors: Mary K Downer; Stacey A Kenfield; Meir J Stampfer; Kathryn M Wilson; Barbra A Dickerman; Edward L Giovannucci; Eric B Rimm; Molin Wang; Lorelei A Mucci; Walter C Willett; June M Chan; Erin L Van Blarigan Journal: J Clin Oncol Date: 2019-04-26 Impact factor: 44.544
Authors: Emrah Yatkin; Lauri Polari; Teemu D Laajala; Annika Smeds; Christer Eckerman; Bjarne Holmbom; Niina M Saarinen; Tero Aittokallio; Sari I Mäkelä Journal: PLoS One Date: 2014-04-03 Impact factor: 3.240
Authors: Justyna Mikuła-Pietrasik; Patrycja Sosińska; Marcin Wierzchowski; Katarzyna Piwocka; Krzysztof Książek Journal: Int J Mol Sci Date: 2013-11-14 Impact factor: 5.923