BACKGROUND: This study presents initial data on presence of nucleostemin--a nucleolar protein typical of stem cells in the normal squamous epithelium of the oropharynx and larynx - in squamous cell carcinoma originating from these epithelia. MATERIALS AND METHODS: Differentiation and proliferation markers such as keratins, beta-catenin, galectin-1, and Ki-67 were studied in parallel with nucleostemin for defining cell characteristics. RESULTS: Nucleostemin was detected in nucleoli of both proliferating basal cells and terminally differentiated suprabasal cells of normal epithelium and in tumor cells. Importantly, malignant transformation was connected with a significant enlargement of nucleostemin-positive nucleoli in these cell types. CONCLUSION: Detection of nucleostemin in head and neck cancer cells, together with the size of nucleoli, may be important in the evaluation of tumor differentiation and biology.
BACKGROUND: This study presents initial data on presence of nucleostemin--a nucleolar protein typical of stem cells in the normal squamous epithelium of the oropharynx and larynx - in squamous cell carcinoma originating from these epithelia. MATERIALS AND METHODS: Differentiation and proliferation markers such as keratins, beta-catenin, galectin-1, and Ki-67 were studied in parallel with nucleostemin for defining cell characteristics. RESULTS:Nucleostemin was detected in nucleoli of both proliferating basal cells and terminally differentiated suprabasal cells of normal epithelium and in tumor cells. Importantly, malignant transformation was connected with a significant enlargement of nucleostemin-positive nucleoli in these cell types. CONCLUSION: Detection of nucleostemin in head and neck cancer cells, together with the size of nucleoli, may be important in the evaluation of tumor differentiation and biology.
Authors: R Yoshida; H Nakayama; M Nagata; A Hirosue; T Tanaka; K Kawahara; Y Nakagawa; Y Matsuoka; J Sakata; H Arita; A Hiraki; M Shinohara; T Ito Journal: Br J Cancer Date: 2014-10-14 Impact factor: 7.640
Authors: Jan Boucek; Tomas Mrkvan; Martin Chovanec; Martin Kuchar; Jaroslav Betka; Vladimir Boucek; Marie Hladikova; Jan Betka; Tomas Eckschlager; Blanka Rihova Journal: J Cell Mol Med Date: 2009-01-14 Impact factor: 5.310