Literature DB >> 17966479

Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples.

Maria Vitale1, Fabrizio Vitale, Stefano Reale, Gesualdo Vesco, Vittoria Currò, Santo Caracappa.   

Abstract

It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.

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Year:  2005        PMID: 17966479

Source DB:  PubMed          Journal:  Rev Cubana Med Trop        ISSN: 0375-0760


  1 in total

1.  Comparison of DNA extraction kits for detection of Burkholderia pseudomallei in spiked human whole blood using real-time PCR.

Authors:  Nicole L Podnecky; Mindy G Elrod; Bruce R Newton; Leslie A Dauphin; Jianrong Shi; Sutthinan Chawalchitiporn; Henry C Baggett; Alex R Hoffmaster; Jay E Gee
Journal:  PLoS One       Date:  2013-02-27       Impact factor: 3.240

  1 in total

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