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Literature DB >> 17965719

Two-photon photostimulation and imaging of neural circuits.

Volodymyr Nikolenko¹, Kira E Poskanzer, Rafael Yuste.

Abstract

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

Entities: Chemical

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Year: 2007 PMID： 17965719 DOI： 10.1038/nmeth1105

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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Cited

100 in total

1. Visual experience-dependent maturation of correlated neuronal activity patterns in a developing visual system.

Authors: Heng Xu; Arseny S Khakhalin; Arto V Nurmikko; Carlos D Aizenman
Journal: J Neurosci Date: 2011-06-01 Impact factor: 6.167

2. Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning.

Authors: Francesca Anselmi; Cathie Ventalon; Aurélien Bègue; David Ogden; Valentina Emiliani
Journal: Proc Natl Acad Sci U S A Date: 2011-11-09 Impact factor: 11.205

3. Astrocytic regulation of cortical UP states.

Authors: Kira E Poskanzer; Rafael Yuste
Journal: Proc Natl Acad Sci U S A Date: 2011-10-25 Impact factor: 11.205

Review 4. Frontiers in optical imaging of cerebral blood flow and metabolism.

Authors: Anna Devor; Sava Sakadžić; Vivek J Srinivasan; Mohammad A Yaseen; Krystal Nizar; Payam A Saisan; Peifang Tian; Anders M Dale; Sergei A Vinogradov; Maria Angela Franceschini; David A Boas
Journal: J Cereb Blood Flow Metab Date: 2012-01-18 Impact factor: 6.200

Two-photon photostimulation and imaging of neural circuits.

1. Visual experience-dependent maturation of correlated neuronal activity patterns in a developing visual system.

2. Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning.

3. Astrocytic regulation of cortical UP states.

Review 4. Frontiers in optical imaging of cerebral blood flow and metabolism.

5. A Bayesian compressed-sensing approach for reconstructing neural connectivity from subsampled anatomical data.

6. Photochemical tools to study dynamic biological processes.

7. Automatic identification of fluorescently labeled brain cells for rapid functional imaging.

8. Intracellular delivery of bioactive molecules using light-addressable nanocapsules.

9. Optogenetics meets optical wavefront shaping.

Review 10. Technologies for imaging neural activity in large volumes.