BACKGROUND: Assays with specificity and cost effectiveness are needed for the measurement of HIV-1 burden to monitor disease progression or response to anti-retroviral therapy (ART) in HIV-1 subtype C infected patients. OBJECTIVES: The objective of this study was to develop and validate an affordable one step real-time RT-PCR assay with high specificity and sensitivity to measure plasma HIV-1 loads in HIV-1 subtype C infected patients. RESULTS: We developed an RT-PCR assay to detect and quantitate plasma HIV-1 levels in HIV-1 subtype C infected patients. An inverse correlation between plasma viral loads (PVL) and CD4+ T-cell numbers was detected at all CDC stages. Significant correlations were found between CD8+ T-cell activation and PVL, as well as with the clinical and immunological status of the patients. CONCLUSIONS: This RT-PCR assay provides a sensitive method to measure PVL in HIV-1 subtype C infected patients. Viral loads correlated with immune activation and can be used to monitor HIV care in India.
BACKGROUND: Assays with specificity and cost effectiveness are needed for the measurement of HIV-1 burden to monitor disease progression or response to anti-retroviral therapy (ART) in HIV-1 subtype C infectedpatients. OBJECTIVES: The objective of this study was to develop and validate an affordable one step real-time RT-PCR assay with high specificity and sensitivity to measure plasma HIV-1 loads in HIV-1 subtype C infectedpatients. RESULTS: We developed an RT-PCR assay to detect and quantitate plasma HIV-1 levels in HIV-1 subtype C infectedpatients. An inverse correlation between plasma viral loads (PVL) and CD4+ T-cell numbers was detected at all CDC stages. Significant correlations were found between CD8+ T-cell activation and PVL, as well as with the clinical and immunological status of the patients. CONCLUSIONS: This RT-PCR assay provides a sensitive method to measure PVL in HIV-1 subtype C infectedpatients. Viral loads correlated with immune activation and can be used to monitor HIV care in India.
Authors: I V Kutyavin; I A Afonina; A Mills; V V Gorn; E A Lukhtanov; E S Belousov; M J Singer; D K Walburger; S G Lokhov; A A Gall; R Dempcy; M W Reed; R B Meyer; J Hedgpeth Journal: Nucleic Acids Res Date: 2000-01-15 Impact factor: 16.971
Authors: Pratip K Chattopadhyay; Daniel C Douek; Stephen J Gange; Karen R Chadwick; Marc Hellerstein; Joseph B Margolick Journal: AIDS Res Hum Retroviruses Date: 2006-06 Impact factor: 2.205
Authors: Priscilla Swanson; Carmen de Mendoza; Yagnya Joshi; Alan Golden; Richard L Hodinka; Vincent Soriano; Sushil G Devare; John Hackett Journal: J Clin Microbiol Date: 2005-08 Impact factor: 5.948
Authors: Ryan C May; Haitao Chu; Joseph G Ibrahim; Michael G Hudgens; Abigail C Lees; David M Margolis Journal: Stat Med Date: 2013-06-20 Impact factor: 2.373
Authors: Ravi Tandon; Maria T M Giret; Devi Sengupta; Vanessa A York; Andrew A Wiznia; Michael G Rosenberg; Esper G Kallas; Lishomwa C Ndhlovu; Douglas F Nixon Journal: PLoS One Date: 2012-09-24 Impact factor: 3.240