Literature DB >> 17960445

Delta6 desaturase mRNA abundance in HepG2 cells is suppressed by unsaturated fatty acids.

Roxanne Portolesi1, Barry C Powell, Robert A Gibson.   

Abstract

The effect of unsaturated fatty acids on the abundance of Delta6 desaturase (D6D) mRNA and the fatty acid composition of HepG2 cell membranes was examined. Supplementation of HepG2 cells with oleic acid (18:1n-9, OA), linoleic acid (18:2n-6, LA), alpha-linolenic acid (18:3n-3, ALA), arachidonic acid (20:4n-6, AA) or eicosapentaenoic acid (20:5n-3, EPA) reduced D6D mRNA abundance by 39 +/- 6.6, 40 +/- 2.2, 31 +/- 5.2, 55 +/- 4.8, and 52 +/- 5.0%, respectively, compared with control cells. Despite the reduction in D6D mRNA abundance, the level of D6D conversion products (20:3n-9, EPA and AA) in OA, ALA and LA supplemented cells, respectively, was elevated above that in control cells. Our results suggest that although unsaturated fatty acids decrease the abundance of D6D mRNA by as much as 50%, the conversion of polyunsaturated fatty acids and accumulation of long chain polyunsaturated fatty acids (LCPUFA) in HepG2 cell phospholipids continues to occur.

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Year:  2007        PMID： 17960445     DOI： 10.1007/s11745-007-3122-9

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  21 in total

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4.  Identification of peroxisome proliferator-responsive human genes by elevated expression of the peroxisome proliferator-activated receptor alpha in HepG2 cells.

Authors:  M H Hsu; U Savas; K J Griffin; E F Johnson
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5.  Changes of the transcriptional and fatty acid profiles in response to n-3 fatty acids in SH-SY5Y neuroblastoma cells.

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Journal:  Lipids       Date:  2005-07       Impact factor: 1.880

6.  cDNA cloning, genomic structure, and chromosomal localization of three members of the human fatty acid desaturase family.

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Journal:  Genomics       Date:  2000-06-01       Impact factor: 5.736

7.  Expression and regulation of delta5-desaturase, delta6-desaturase, stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase 2 in rat testis.

Authors:  Thomas Saether; Thien N Tran; Helge Rootwelt; Bjørn O Christophersen; Trine B Haugen
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8.  The E-box like sterol regulatory element mediates the suppression of human Delta-6 desaturase gene by highly unsaturated fatty acids.

Authors:  Takayuki Y Nara; Wei Song He; Chongren Tang; Steven D Clarke; Manabu T Nakamura
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Review 9.  Peroxisome proliferator-activated receptors and the regulation of mammalian lipid metabolism.

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Journal:  Biochem Soc Trans       Date:  2002-11       Impact factor: 5.407

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Authors:  Manabu T Nakamura; Takayuki Y Nara
Journal:  Annu Rev Nutr       Date:  2004       Impact factor: 11.848
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2.  Inhibition of MEK/ERK1/2 Signaling Affects the Fatty Acid Composition of HepG2 Human Hepatic Cell Line.

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4.  Effects of FADS and ELOVL polymorphisms on indexes of desaturase and elongase activities: results from a pre-post fish oil supplementation.

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5.  Loss of FADS2 function severely impairs the use of HeLa cells as an in vitro model for host response studies involving fatty acid effects.

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  5 in total

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