Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells.

Cytokeratin 1, an intermediate filament keratin, was isolated as a partner of the tyrosine kinase Src from neuroblastoma NMB7 cells. The cytokeratin 1-Src complex was found to be associated with the molecular scaffolder RACK1 (receptor for activated protein kinase C). Interestingly, the cytokeratin 1-Src-RACK1 complex was found to actively bind with membrane receptors such as integrin beta1. We are interested in using this complex to find downstream kinases and phosphatases that bind upon cytokine stimulation, especially during neurogenesis.