Literature DB >> 17952088

STED microscopy with continuous wave beams.

Katrin I Willig1, Benjamin Harke, Rebecca Medda, Stefan W Hell.   

Abstract

We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.

Mesh：

Substances：

Year:  2007        PMID： 17952088     DOI： 10.1038/nmeth1108

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  89 in total

1.  Two-color STED microscopy of living synapses using a single laser-beam pair.

Authors:  Jan Tønnesen; Fabien Nadrigny; Katrin I Willig; Roland Wedlich-Söldner; U Valentin Nägerl
Journal:  Biophys J       Date:  2011-11-15       Impact factor: 4.033

2.  All-optical fluorescence image recovery using modulated Stimulated Emission Depletion.

Authors:  Chaoyang Fan; Jung-Cheng Hsiang; Amy E Jablonski; Robert M Dickson
Journal:  Chem Sci       Date:  2011       Impact factor: 9.825

3.  Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging.

Authors:  Paolo Bianchini; Benjamin Harke; Silvia Galiani; Giuseppe Vicidomini; Alberto Diaspro
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-09       Impact factor: 11.205

4.  STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein.

Authors:  Lana Lau; Yin Loon Lee; Steffen J Sahl; Tim Stearns; W E Moerner
Journal:  Biophys J       Date:  2012-06-19       Impact factor: 4.033

5.  Light-guided surface engineering for biomedical applications.

Authors:  Ashwath Jayagopal; Gregory P Stone; Frederick R Haselton
Journal:  Bioconjug Chem       Date:  2008-03-04       Impact factor: 4.774

6.  Analytical description of high-aperture STED resolution with 0-2π vortex phase modulation.

Authors:  Hao Xie; Yujia Liu; Dayong Jin; Philip J Santangelo; Peng Xi
Journal:  J Opt Soc Am A Opt Image Sci Vis       Date:  2013-08-01       Impact factor: 2.129

7.  Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell.

Authors:  Birka Hein; Katrin I Willig; Stefan W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-16       Impact factor: 11.205

Review 8.  Spatial quantitative analysis of fluorescently labeled nuclear structures: problems, methods, pitfalls.

Authors:  O Ronneberger; D Baddeley; F Scheipl; P J Verveer; H Burkhardt; C Cremer; L Fahrmeir; T Cremer; B Joffe
Journal:  Chromosome Res       Date:  2008       Impact factor: 5.239

Review 9.  Do-it-yourself guide: how to use the modern single-molecule toolkit.

Authors:  Nils G Walter; Cheng-Yen Huang; Anthony J Manzo; Mohamed A Sobhy
Journal:  Nat Methods       Date:  2008-06       Impact factor: 28.547

10.  Membrane microdomains and cytoskeleton organization shape and regulate the IL-7 receptor signalosome in human CD4 T-cells.

Authors:  Blanche Tamarit; Florence Bugault; Anne-Hélène Pillet; Vincent Lavergne; Pascal Bochet; Nathalie Garin; Ulf Schwarz; Jacques Thèze; Thierry Rose
Journal:  J Biol Chem       Date:  2013-01-17       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.