SNPbox: web-based high-throughput primer design with an eye for repetitive sequences.

In this omics era, researchers in biosciences need more than ever user-friendly, fast, and straightforward computational tools for high-throughput research. In this chapter, we present SNPbox and explain the general primer design strategy. We also show how the four modules, Exon, Single-Nucleotide Polymorphism (SNP), Saturation, and Exon + Saturation, exactly apply the primer design strategy, give clear guidance for users of the Web interface at http://www.SNPbox.org, and explain how pre-designed primers for Ensembl genes can be visualized and retrieved.