| Literature DB >> 17951791 |
Abstract
The conflicts between several design objectives for PCR primers are computationally resolved by specifying a set of ideal parameters and searching for primer pairs whose parameters approximate this ideal point as close as possible. It thus becomes feasible to identify an "optimum" and to efficiently compute it. User-specified target regions, primer conditions, and procedural conditions are obeyed.Mesh：
Substances：
Year: 2007 PMID： 17951791 DOI： 10.1007/978-1-59745-528-2_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745