| Literature DB >> 17951647 |
Jean-Claude Farre1, Kanae Shirahama-Noda, Lan Zhang, Keith Booher, Suresh Subramani.
Abstract
This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.Entities:
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Year: 2007 PMID： 17951647 DOI： 10.1007/978-1-59745-456-8_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745