Literature DB >> 17947593

Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF.

Lakshmi Kannan1, Narayan C Rath, Rohana Liyanage, Jackson O Lay.   

Abstract

The aim of the study was to determine chicken monocyte- and granulocyte-associated peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purification and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin beta-4 (Tbeta-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to determine the modulation of Tbeta-4 gene expression or its secretion, showed no changes.

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Year:  2007        PMID： 17947593     DOI： 10.1196/annals.1415.028

Source DB:  PubMed          Journal:  Ann N Y Acad Sci        ISSN： 0077-8923            Impact factor:   5.691


  8 in total

1.  Theory of the Protein Equilibrium Population Snapshot by H/D Exchange Electrospray Ionization Mass Spectrometry (PEPS-HDX-ESI-MS) Method used to obtain Protein Folding Energies/Rates and Selected Supporting Experimental Evidence.

Authors:  Rohana Liyanage; Nagarjuna Devarapalli; Derek B Pyland; Latisha M Puckett; N H Phan; Joel A Starch; Mark R Okimoto; Jennifer Gidden; Wesley E Stites; Jackson O Lay
Journal:  Int J Mass Spectrom       Date:  2012-10-23       Impact factor: 1.986

2.  Effect of toll-like receptor activation on thymosin beta-4 production by chicken macrophages.

Authors:  Lakshmi Kannan; Narayan C Rath; Rohana Liyanage; Jackson O Lay
Journal:  Mol Cell Biochem       Date:  2010-07-08       Impact factor: 3.396

3.  In-feed supplementation of trans-cinnamaldehyde reduces layer-chicken egg-borne transmission of Salmonella enterica serovar enteritidis.

Authors:  Indu Upadhyaya; Abhinav Upadhyay; Anup Kollanoor-Johny; Shankumar Mooyottu; Sangeetha A Baskaran; Hsin-Bai Yin; David T Schreiber; Mazhar I Khan; Michael J Darre; Patricia A Curtis; Kumar Venkitanarayanan
Journal:  Appl Environ Microbiol       Date:  2015-02-20       Impact factor: 4.792

4.  Expression pattern of thymosin beta 4 in the adult human liver.

Authors:  S Nemolato; P Van Eyken; T Cabras; F Cau; M U Fanari; A Locci; D Fanni; C Gerosa; I Messana; M Castagnola; G Faa
Journal:  Eur J Histochem       Date:  2011-08-27       Impact factor: 3.188

5.  Immunoreactivity of thymosin beta 4 in human foetal and adult genitourinary tract.

Authors:  S Nemolato; T Cabras; M U Fanari; F Cau; D Fanni; C Gerosa; B Manconi; I Messana; M Castagnola; G Faa
Journal:  Eur J Histochem       Date:  2010-10-13       Impact factor: 3.188

6.  Lactobacillus bulgaricus, Lactobacillus rhamnosus and Lactobacillus paracasei Attenuate Salmonella Enteritidis, Salmonella Heidelberg and Salmonella Typhimurium Colonization and Virulence Gene Expression In Vitro.

Authors:  Muhammed Shafeekh Muyyarikkandy; Mary Anne Amalaradjou
Journal:  Int J Mol Sci       Date:  2017-11-09       Impact factor: 5.923

7.  DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant.

Authors:  Adil S Al-Ogaili; Rohana Liyanage; Jack O Lay; Tieshan Jiang; Christine N Vuong; Shilpi Agrawal; Thallapuranam Krishnaswamy Suresh Kumar; Luc R Berghman; Billy M Hargis; Young Min Kwon
Journal:  Sci Rep       Date:  2020-12-17       Impact factor: 4.379

8.  Effect of plant derived antimicrobials on Salmonella enteritidis adhesion to and invasion of primary chicken oviduct epithelial cells in vitro and virulence gene expression.

Authors:  Indu Upadhyaya; Abhinav Upadhyay; Anup Kollanoor-Johny; Michael J Darre; Kumar Venkitanarayanan
Journal:  Int J Mol Sci       Date:  2013-05-21       Impact factor: 5.923
  8 in total

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