Literature DB >> 1794109

The clastogenic effect of pyrimethamine (Daraprim) on human chromosomes in lymphocyte cultures.

U Egeli1, G Erdogan.   

Abstract

In this study the clastogenic effect of pyrimethamine (Daraprim), a folic acid antagonist used for the treatment of toxoplasmosis and malaria on human chromosomes, was investigated. Pyrimethamine was added to in vitro lymphocyte cultures at six different concentrations: 0.05 (normal therapeutic dose), 0.1, 0.2, 0.4, 0.8, and 1.6 mg/ml. No proliferation was observed in any of the cultures containing 1.6 mg/ml pyrimethamine. The results of the cytogenetic evaluations show that the frequency of breaks and gaps increase significantly in dose-dependent manner. Thus, pyrimethamine has a clastogenic effect on human chromosomes.

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Year:  1991        PMID： 1794109     DOI： 10.1007/BF00124070

Source DB:  PubMed          Journal:  Cell Biol Toxicol        ISSN： 0742-2091            Impact factor:   6.691


  9 in total

1.  Heritable fragile sites on human chromosomes I. Factors affecting expression in lymphocyte culture.

Authors:  G R Sutherland
Journal:  Am J Hum Genet       Date:  1979-03       Impact factor: 11.025

2.  Mechanism of pyrimethamine-induced megaloblastosis in human bone marrow.

Authors:  S Waxman; V Herbert
Journal:  N Engl J Med       Date:  1969-06-12       Impact factor: 91.245

3.  Chromosome breakage and recombination at fragile sites.

Authors:  T W Glover; C K Stein
Journal:  Am J Hum Genet       Date:  1988-09       Impact factor: 11.025

4.  In vitro antimalarial activity of tetrahydrofolate dehydrogenase inhibitors.

Authors:  D G Sixsmith; W M Watkins; J D Chulay; H C Spencer
Journal:  Am J Trop Med Hyg       Date:  1984-09       Impact factor: 2.345

Review 5.  The fragile X chromosome.

Authors:  G R Sutherland
Journal:  Int Rev Cytol       Date:  1983

6.  Fragile sites in chromosomes: possible model for the study of spontaneous chromosome breakage.

Authors:  P B Jacky; B Beek; G R Sutherland
Journal:  Science       Date:  1983-04-01       Impact factor: 47.728

7.  Folic acid and chromosome breakage. II. A methionine effect similar to that in fragile X expression.

Authors:  J A Reidy; A T Chen
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

8.  Folic acid and chromosome breakage. I. Implications for genotoxicity studies.

Authors:  J A Reidy; X Zhou; A T Chen
Journal:  Mutat Res       Date:  1983-11       Impact factor: 2.433

9.  Chromosomal breakage in normal and fragile X subjects using low folate culture conditions.

Authors:  M Vekemans; B Popovich; D Rosenblatt; P Monroe
Journal:  J Med Genet       Date:  1983-12       Impact factor: 6.318
  9 in total
  2 in total

1.  Evaluation of the effect of pyrimethamine, an anti-malarial drug, on HIV-1 replication.

Authors:  Raphael M Oguariri; Joseph W Adelsberger; Michael W Baseler; Tomozumi Imamichi
Journal:  Virus Res       Date:  2010-08-26       Impact factor: 3.303

2.  ECVAM retrospective validation of in vitro micronucleus test (MNT).

Authors:  Raffaella Corvi; Silvio Albertini; Thomas Hartung; Sebastian Hoffmann; Daniela Maurici; Stefan Pfuhler; Jan van Benthem; Philippe Vanparys
Journal:  Mutagenesis       Date:  2008-03-07       Impact factor: 3.000
  2 in total

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