| Literature DB >> 17940876 |
Xiaojing Wang1, Honghua Zheng, Changjin Liu, Changgeng Zhu, Wei Wang, Zhengli Li.
Abstract
Astrocytes are activated by ciliary neurotrophic factor (CNTF) in vivo and in vitro, however, the consequences on the L-type calcium channel (LCC) of neurons are still poorly understood. Therefore, in the present study, whole-cell patch clamp, western-blot and RT-PCR assay were performed to evaluate the effects of CNTF-treated astrocyte conditioned medium (CNTF-ACM) on LCC current (I(Ca)-L) and the expression of Cav1.2 and Cav1.3 in Sprague-Dawley rat cortical neurons. The results revealed that CNTF-ACM enhanced the amplitude of Ica-L and the expression of Cav1.3 significantly, but had no effects on Cav1.2 expression. We also found an increase in the concentration of fibroblast growth factor-2 (FGF-2) in CNTF-ACM by ELISA assay. Taken together, these findings indicate that CNTF induces the release of factors, including FGF-2, from astrocytes, thereby potentiating the activity of LCC in cortical neurons.Entities:
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Year: 2007 PMID： 17940876 DOI： 10.1007/s11064-007-9514-7
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996