U-D Braumann1, N Scherf, J Einenkel, L-C Horn, N Wentzensen, M Loeffler, J-P Kuska. 1. University of Leipzig, Translational Centre for Regenerative Medicine, Department of Obstetrics and Gynecology, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany. braumann@uni-leipzig.de
Abstract
OBJECTIVES: A proof of principle study was conducted for microscopic tissue volume reconstructions using a new image processing chain operating on alternately stained large histological serial sections. METHODS: Digital histological images were obtained from conventional brightfield transmitted light microscopy. A powerful nonparametric nonlinear optical flow-based registration approach was used. In order to apply a simple but computationally feasible sum-of-squared-differences similarity measure even in case of differing histological stainings, a new consistent tissue segmentation procedure was placed upstream. RESULTS: Two reconstructions from uterine cervix carcinoma specimen were accomplished, one alternately stained with p16(INK4a) (surrogate tumor marker) and H&E (routine reference), and another with three different alternate stainings, H&E, p16(INK4a), and CD3 (a T-lymphocyte marker). For both cases, due to our segmentation-based reference-free nonlinear registration procedure, resulting tissue reconstructions exhibit utmost smooth image-to-image transitions without impairing warpings. CONCLUSIONS: Our combination of modern nonparametric nonlinear registration and consistent tissue segmentation has turned out to provide a superior tissue reconstruction quality.
OBJECTIVES: A proof of principle study was conducted for microscopic tissue volume reconstructions using a new image processing chain operating on alternately stained large histological serial sections. METHODS: Digital histological images were obtained from conventional brightfield transmitted light microscopy. A powerful nonparametric nonlinear optical flow-based registration approach was used. In order to apply a simple but computationally feasible sum-of-squared-differences similarity measure even in case of differing histological stainings, a new consistent tissue segmentation procedure was placed upstream. RESULTS: Two reconstructions from uterine cervix carcinoma specimen were accomplished, one alternately stained with p16(INK4a) (surrogate tumor marker) and H&E (routine reference), and another with three different alternate stainings, H&E, p16(INK4a), and CD3 (a T-lymphocyte marker). For both cases, due to our segmentation-based reference-free nonlinear registration procedure, resulting tissue reconstructions exhibit utmost smooth image-to-image transitions without impairing warpings. CONCLUSIONS: Our combination of modern nonparametric nonlinear registration and consistent tissue segmentation has turned out to provide a superior tissue reconstruction quality.
Authors: Jasmine N Singh; Taylor M Nowlin; Gregory J Seedorf; Steven H Abman; Douglas P Shepherd Journal: J Biomed Opt Date: 2017-07-01 Impact factor: 3.170
Authors: Monica L Berlanga; Sébastien Phan; Eric A Bushong; Stephanie Wu; Ohkyung Kwon; Binh S Phung; Steve Lamont; Masako Terada; Tolga Tasdizen; Maryann E Martone; Mark H Ellisman Journal: Front Neuroanat Date: 2011-03-07 Impact factor: 3.856