Literature DB >> 1793799

High-performance liquid chromatography with electrochemical detection for determination of the major malondialdehyde-guanine adduct.

Y Goda1, L J Marnett.   

Abstract

A method is described for quantitative analysis of the pyrimidopurinone adduct (M1G) arising from reaction of malondialdehyde (MDA) with DNA. DNA samples treated with MDA are reduced with sodium borohydride and hydrolyzed with 0.1 N HCl. The bases released are isolated by solid-phase extraction and analyzed by high-performance liquid chromatography with electrochemical detection. 5,6-Dihydro-M1G (H2M1G) is detected with a glassy carbon electrode at an applied potential of 700 mV vs Ag/AgCl. At this potential, interference from normal deoxynucleoside bases is low. The limit of detection of H2M1G eluted from an HPLC column is 100-200 fmol. The method described should be useful for quantitation of M1G in a variety of DNA samples and biological fluids.

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Year:  1991        PMID： 1793799     DOI： 10.1021/tx00023a005

Source DB:  PubMed          Journal:  Chem Res Toxicol        ISSN： 0893-228X            Impact factor:   3.739


  11 in total

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2.  Malondialdehyde-deoxyguanosine and bulky DNA adducts in schoolchildren resident in the proximity of the Sarroch industrial estate on Sardinia Island, Italy.

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Review 3.  Generation of mutagens during arachidonic acid metabolism.

Authors:  L J Marnett
Journal:  Cancer Metastasis Rev       Date:  1994-12       Impact factor: 9.264

4.  Aberrant methylation of hypermethylated-in-cancer-1 and exocyclic DNA adducts in tobacco smokers.

Authors:  Marco E M Peluso; Armelle Munnia; Valentina Bollati; Petcharin Srivatanakul; Adisorn Jedpiyawongse; Suleeporn Sangrajrang; Marcello Ceppi; Roger W Giese; Paolo Boffetta; Andrea A Baccarelli
Journal:  Toxicol Sci       Date:  2013-10-23       Impact factor: 4.849

5.  Fruit and vegetable and fried food consumption and 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α] purin-10(3H)-one deoxyguanosine adduct formation.

Authors:  Marco Peluso; Armelle Munnia; Sara Piro; Adisorn Jedpiyawongse; Suleeporn Sangrajrang; Roger W Giese; Marcello Ceppi; Paolo Boffetta; Petcharin Srivatanakul
Journal:  Free Radic Res       Date:  2011-12-01

6.  Breast fine-needle aspiration malondialdehyde deoxyguanosine adduct in breast cancer.

Authors:  Marco Peluso; Armelle Munnia; Gabriella G Risso; Sandra Catarzi; Sara Piro; Marcello Ceppi; Roger W Giese; Beniamino Brancato
Journal:  Free Radic Res       Date:  2011-01-21

7.  Malondialdehyde-deoxyguanosine adduct formation in workers of pathology wards: the role of air formaldehyde exposure.

Authors:  Roberto Bono; Valeria Romanazzi; Armelle Munnia; Sara Piro; Alessandra Allione; Fulvio Ricceri; Simonetta Guarrera; Cristina Pignata; Giuseppe Matullo; Poguang Wang; Roger W Giese; Marco Peluso
Journal:  Chem Res Toxicol       Date:  2010-08-16       Impact factor: 3.739

8.  Immunological approach to investigating membrane cell damages induced by lipoperoxidative stress. Application to far UV-irradiated erythrocytes.

Authors:  E Petit; D Divoux; Y Chancerelle; J F Kergonou; A Nouvelot
Journal:  Biol Trace Elem Res       Date:  1995 Jan-Mar       Impact factor: 3.738

Review 9.  Cigarette smoke radicals and the role of free radicals in chemical carcinogenicity.

Authors:  W A Pryor
Journal:  Environ Health Perspect       Date:  1997-06       Impact factor: 9.031

10.  Exocycilic DNA Adducts in a Murine Model of Non-alcoholic Steatohepatitis.

Authors:  Marco E M Peluso; Armelle Munnia; Mirko Tarocchi; Mario Arciello; Clara Balsano; Roger W Giese; Andrea Galli
Journal:  J Carcinog Mutagen       Date:  2013-10-18
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