David K Meyerholz1, Isaac Samuel. 1. Department of Pathology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USA.
Abstract
BACKGROUND: Bile-pancreatic duct ligation in rats causes acute pancreatic inflammation. We performed serial morphologic evaluation of the exocrine pancreas after duct ligation to facilitate further investigations using the model. METHODS: The pancreas was excised from 74 rats after 0, 1, 3, 5, 24 or 48 hours of duct ligation or sham surgery. A pathologist evaluated 1 hematoxylin- and eosin-stained slide from each rat. Confirmatory immunostaining was performed with markers for apoptosis (activated caspase-3), proliferation (cyclin D3), neutrophils (myeloperoxidase), and macrophages (CD68). RESULTS: Interstitial edema and white blood cell infiltration were apparent at 24 hours and increased at 48 hours. Progressive periods of duct ligation were characterized by ductular ectasia (1 to 3 hours), acinar vacuolization (5 to 48 hours), leukocytic margination and neutrophil exocytosis (5 to 48 hours), ductule epithelium hypertrophy and proliferation (24 to 48 hours), and discernible loss of zymogen granules (48 hours). CONCLUSIONS: Ligation-induced acute pancreatitis in rats is a useful model to investigate early events in disease pathogenesis.
BACKGROUND:Bile-pancreatic duct ligation in rats causes acute pancreatic inflammation. We performed serial morphologic evaluation of the exocrine pancreas after duct ligation to facilitate further investigations using the model. METHODS: The pancreas was excised from 74 rats after 0, 1, 3, 5, 24 or 48 hours of duct ligation or sham surgery. A pathologist evaluated 1 hematoxylin- and eosin-stained slide from each rat. Confirmatory immunostaining was performed with markers for apoptosis (activated caspase-3), proliferation (cyclin D3), neutrophils (myeloperoxidase), and macrophages (CD68). RESULTS: Interstitial edema and white blood cell infiltration were apparent at 24 hours and increased at 48 hours. Progressive periods of duct ligation were characterized by ductular ectasia (1 to 3 hours), acinar vacuolization (5 to 48 hours), leukocytic margination and neutrophil exocytosis (5 to 48 hours), ductule epithelium hypertrophy and proliferation (24 to 48 hours), and discernible loss of zymogen granules (48 hours). CONCLUSIONS: Ligation-induced acute pancreatitis in rats is a useful model to investigate early events in disease pathogenesis.
Authors: Marc J Berna; K Martin Hoffmann; Jose A Tapia; Michelle Thill; Andrea Pace; Samuel A Mantey; Robert T Jensen Journal: Biochim Biophys Acta Date: 2006-12-24
Authors: Nandita Raikwar; Cameron Braverman; Peter M Snyder; Robert A Fenton; David K Meyerholz; Francois M Abboud; Sailesh C Harwani Journal: Am J Physiol Heart Circ Physiol Date: 2019-06-07 Impact factor: 4.733
Authors: Gary W Hunninghake; Kevin C Doerschug; Amanda B Nymon; Gregory A Schmidt; David K Meyerholz; Alix Ashare Journal: Am J Respir Crit Care Med Date: 2010-04-22 Impact factor: 21.405
Authors: Sailesh C Harwani; Jason Ratcliff; Fayyaz S Sutterwala; Zuhair K Ballas; David K Meyerholz; Mark W Chapleau; Francois M Abboud Journal: Circ Res Date: 2016-09-22 Impact factor: 17.367
Authors: Duraisamy Kempuraj; Erik C Twait; Deborah E Williard; Zuobiao Yuan; David K Meyerholz; Isaac Samuel Journal: PLoS One Date: 2013-02-13 Impact factor: 3.240