| Literature DB >> 17929150 |
Flávia Rodrigues de Oliveira Silva1, Ricardo Elgul Samad, Laércio Gomes, Lilia Coronato Courrol.
Abstract
We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H(2)O(2), the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.Entities:
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Year: 2007 PMID: 17929150 DOI: 10.1007/s10895-007-0260-7
Source DB: PubMed Journal: J Fluoresc ISSN: 1053-0509 Impact factor: 2.217