| Literature DB >> 17926073 |
Tina Lütke-Eversloh1, Gregory Stephanopoulos.
Abstract
In this study, we developed a fluorescence-based assay for quantifying the aromatic amino acid L-tyrosine in small sample volumes employing 96-well microtiter plates. The method was based on the specific derivatisation of L-tyrosine with 1-nitroso-2-naphthol and the formation of a stable complex for the fluorescent determination of L-tyrosine concentrations. The original procedure for L-tyrosine measurements in blood or tissue samples (Waalkes and Udenfriend in J Lab Clin Med 50:733-736, 1957) was modified to a simple assay suitable for high-throughput screening of L-tyrosine producing microorganisms such as engineered Escherichia coli.Entities:
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Year: 2007 PMID： 17926073 DOI： 10.1007/s10295-007-0257-x
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346