The involvement of a P38-like MAP kinase in ABA-induced and H2O2-mediated stomatal closure in Vicia faba L.

SB203580 is a specific inhibitor of p38 mitogen-activated protein (MAP) kinase and has been widely used to investigate the physiological roles of p38 in animal and yeast cells. Here by using an epidermal strip bioassay, laser-scanning confocal microscopy and whole-cell patch clamp analysis, we assess the effects of pyridinyl imidazoles-like SB203580 on the H(2)O(2) signaling in guard cells of Vicia faba L. The results indicated that SB203580 blocks H(2)O(2)- or ABA-induced stomatal closure, ABA-induced H(2)O(2) generation, and decrease in K(+) fluxing across plasma membrane of Vicia guard cells by application of ABA and H(2)O(2), whereas its analog SB202474 had no effect on these events. Thus, these results suggest that activation of p38-like MAP kinase modulates guard cell ROS signaling in response to stress.