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Literature DB >> 17923236

Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays.

Abstract

The sedimentation of an mRNA in sucrose gradients is highly affected by its ribosomal association. Sedimentation analysis has therefore become routine for studying changes in ribosomal association of mRNAs of interest. DNA microarray technology has been combined with sedimentation analysis to characterize changes in ribosomal association for thousands of mRNAs in parallel. Such analyses revealed mRNAs that are translationally regulated and have provided new insights into the translation process. In this chapter, we describe possible experimental designs for analyzing genome-wide changes in ribosomal association, and discuss some of their advantages and disadvantages. We then provide a detailed protocol for analysis of polysomal fractions using spotted DNA microarrays.

Entities: Chemical

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Year: 2007 PMID： 17923236 DOI： 10.1016/S0076-6879(07)31010-0

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

19 in total

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Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays.

Review 1. miRNAs: effectors of environmental influences on gene expression and disease.

2. Yeast translational response to high salinity: global analysis reveals regulation at multiple levels.

3. Translational landscape of photomorphogenic Arabidopsis.

4. Calorie restriction reprograms diurnal rhythms in protein translation to regulate metabolism.

5. Isolation of mRNAs associated with yeast mitochondria to study mechanisms of localized translation.

6. Multimodal RNA-seq using single-strand, double-strand, and CircLigase-based capture yields a refined and extended description of the C. elegans transcriptome.

Review 7. Translation regulation gets its 'omics' moment.

8. Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner.

9. Stress-dependent coordination of transcriptome and translatome in yeast.

Review 10. RNA-seq: from technology to biology.