| Literature DB >> 17918875 |
Bart Ghesquière1, Lien Buyl, Hans Demol, Jozef Van Damme, An Staes, Evy Timmerman, Joël Vandekerckhove, Kris Gevaert.
Abstract
A new approach for proteome-wide analysis of sialylated N-glycopeptides based on the diagonal chromatographic COFRADIC technology is presented here. The use of alpha(2-3,6,8,9) neuraminidase is central to isolate sialylated N-glycopeptides out of a complex peptide mixture. Two different COFRADIC techniques are introduced here, either without or with post-metabolic oxygen-18 labeling (direct versus indirect sorting), and when applied to immuno-depleted mouse serum, we herewith identified 93 sialylated glycosylation sites in 53 serum proteins.Entities:
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Year: 2007 PMID： 17918875 DOI： 10.1021/pr0703728
Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466