PURPOSE: To evaluate the efficacy of topical voriconazole in an experimental rabbit model of Fusarium keratitis. METHODS: Fungal keratitis was induced in the right eyes of 24 New Zealand rabbits. 8.6 x 10(3) CFU/0.1 ml F.solani spore suspension was injected midstromally into the central cornea. Group 1 received topical amphotericin B 0.15%, group 2 received topical itraconazole 1% and group 3 received topical voriconazole 1% hourly between 08:00 to 22:00 on days 1 and 2; 4 times daily on days 3-5. Control group received topical balanced salt solution at identical intervals. The eyes were examined clinically with a scoring system before treatment (day 0), on day 3 and on day 5. Cultures were taken from the lesion by scraping at the end of the treatment. Clinical scores and microbiologic results were analyzed statistically. RESULTS: In the control group, keratitis progressed clinically and colony level was 2 x 10(3) CFU at day 5. In all treatment groups, progression of keratitis was inhibited clinically. Culture was sterile in the group receiving amphotericin B. Colony level was 0.3 x 10(2) CFU in the itraconazole group and 2 x 10(2) CFU in the voriconazole group at day 5. CONCLUSION: Progression of keratitis was inhibited clinically in all treatment groups. Colony level decreased significantly in all treatment groups. As a result, itraconazole 1% and voriconazole 1% were found to be effective in Fusarium keratitis clinically and microbiologically, although their activity was not as effective as amphotericin B 0.15%.
PURPOSE: To evaluate the efficacy of topical voriconazole in an experimental rabbit model of Fusariumkeratitis. METHODS:Fungal keratitis was induced in the right eyes of 24 New Zealand rabbits. 8.6 x 10(3) CFU/0.1 ml F.solani spore suspension was injected midstromally into the central cornea. Group 1 received topical amphotericin B 0.15%, group 2 received topical itraconazole 1% and group 3 received topical voriconazole 1% hourly between 08:00 to 22:00 on days 1 and 2; 4 times daily on days 3-5. Control group received topical balanced salt solution at identical intervals. The eyes were examined clinically with a scoring system before treatment (day 0), on day 3 and on day 5. Cultures were taken from the lesion by scraping at the end of the treatment. Clinical scores and microbiologic results were analyzed statistically. RESULTS: In the control group, keratitis progressed clinically and colony level was 2 x 10(3) CFU at day 5. In all treatment groups, progression of keratitis was inhibited clinically. Culture was sterile in the group receiving amphotericin B. Colony level was 0.3 x 10(2) CFU in the itraconazole group and 2 x 10(2) CFU in the voriconazole group at day 5. CONCLUSION: Progression of keratitis was inhibited clinically in all treatment groups. Colony level decreased significantly in all treatment groups. As a result, itraconazole 1% and voriconazole 1% were found to be effective in Fusariumkeratitis clinically and microbiologically, although their activity was not as effective as amphotericin B 0.15%.
Authors: Rocus R Klont; Cathrien A Eggink; Antonius J M M Rijs; Pieter Wesseling; Paul E Verweij Journal: Clin Infect Dis Date: 2005-05-12 Impact factor: 9.079
Authors: Marlene L Durand; Ivana K Kim; Donald J D'Amico; John I Loewenstein; Ellis H Tobin; Shalom J Kieval; Stephen S Martin; Dimitri T Azar; Frederick S Miller; Brandon J Lujan; Joan W Miller Journal: Am J Ophthalmol Date: 2005-09 Impact factor: 5.258
Authors: A K Leck; P A Thomas; M Hagan; J Kaliamurthy; E Ackuaku; M John; M J Newman; F S Codjoe; J A Opintan; C M Kalavathy; V Essuman; C A N Jesudasan; G J Johnson Journal: Br J Ophthalmol Date: 2002-11 Impact factor: 4.638
Authors: Wolfram Schreiber; Antje Olbrisch; Christian K Vorwerk; Wolfgang König; Wolfgang Behrens-Baumann Journal: Invest Ophthalmol Vis Sci Date: 2003-06 Impact factor: 4.799
Authors: Christian K Vorwerk; F Streit; L Binder; S Tuchen; C Knop; W Behrens-Baumann Journal: Graefes Arch Clin Exp Ophthalmol Date: 2008-05-20 Impact factor: 3.117
Authors: Renata T Kashiwabuchi; Fabio R S Carvalho; Yasin A Khan; Flavio Hirai; Mauro S Campos; Peter J McDonnell Journal: Graefes Arch Clin Exp Ophthalmol Date: 2012-11-25 Impact factor: 3.117
Authors: Thet Tun Aung; Wei Hong Jeff Chor; Myoe Naing Lynn; Anita Sook Yee Chan; Donald T H Tan; Roger W Beuerman Journal: Eye Contact Lens Date: 2020-09 Impact factor: 3.152