Literature DB >> 1791161

DNA fluorescence induced by polymethine cation pyrvinium binding.

J C Stockert1, C I Trigoso, A R Llorente, P Del Castillo.   

Abstract

Pyrvinium is a polymethine cation which shows interesting fluorescence emission and DNA binding properties. In diluted aqueous solution, pyrvinium pamoate induced a bright yellow fluorescence in kinetoplast DNA from Trypanosoma cruzi epimastigotes as well as in chicken erythrocyte nuclei under a wide range of excitations. No fading was observed after mounting in suitable media. Spectroscopic studies on pyrvinium solutions revealed bathochromic and hypochromic shifts in the absorption spectrum of its complex with DNA. A striking enhancement of pyrvinium fluorescence was found in solvents of high viscosity or after binding to DNA. Experimental results and the chemical structure of pyrvinium allow us to suggest that the minor groove of adenine-thymine DNA regions could be the specific binding site for this new DNA fluorochrome.

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Year:  1991        PMID： 1791161     DOI： 10.1007/bf01041182

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  22 in total

1.  [Fluorescence of auramine O in the presence of nucleic acid].

Authors:  G OSTER
Journal:  C R Hebd Seances Acad Sci       Date:  1951-04-30

Review 2.  Nonintercalating DNA-binding ligands: specificity of the interaction and their use as tools in biophysical, biochemical and biological investigations of the genetic material.

Authors:  C Zimmer; U Wähnert
Journal:  Prog Biophys Mol Biol       Date:  1986       Impact factor: 3.667

3.  New fluorescence reactions in DNA cytochemistry. 1. Microscopic and spectroscopic studies on nonrigid fluorochromes.

Authors:  J C Stockert; P Del Castillo; A R Llorente; D M Rasskin; J B Romero; A Gómez
Journal:  Anal Quant Cytol Histol       Date:  1990-02       Impact factor: 0.302

4.  Hydroxystilbamidine. A nonintercalating drug as a probe of nucleic acid conformation.

Authors:  B Festy; M Daune
Journal:  Biochemistry       Date:  1973-11-20       Impact factor: 3.162

5.  Fluorescence of free and protein-bound auramine O.

Authors:  R F Chen
Journal:  Arch Biochem Biophys       Date:  1977-03       Impact factor: 4.013

6.  Interactions of antitumor drugs with natural DNA: 1H NMR study of binding mode and kinetics.

Authors:  J Feigon; W A Denny; W Leupin; D R Kearns
Journal:  J Med Chem       Date:  1984-04       Impact factor: 7.446

7.  Theoretical studies of the selective binding to DNA of two non-intercalating ligands: netropsin and SN 18071.

Authors:  K Zakrzewska; R Lavery; B Pullman
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

8.  Molecular recognition between oligopeptides and nucleic acids: novel imidazole-containing oligopeptides related to netropsin that exhibit altered DNA sequence specificity.

Authors:  J W Lown; K Krowicki; U G Bhat; A Skorobogaty; B Ward; J C Dabrowiak
Journal:  Biochemistry       Date:  1986-11-18       Impact factor: 3.162

9.  Unique poly(dA).poly(dT) B'-conformation in cellular and synthetic DNAs.

Authors:  S Diekmann; D A Zarling
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

10.  A bifurcated hydrogen-bonded conformation in the d(A.T) base pairs of the DNA dodecamer d(CGCAAATTTGCG) and its complex with distamycin.

Authors:  M Coll; C A Frederick; A H Wang; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205
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  4 in total

1.  The FDA-Approved Anthelmintic Pyrvinium Pamoate Inhibits Pancreatic Cancer Cells in Nutrient-Depleted Conditions by Targeting the Mitochondria.

Authors:  Christopher W Schultz; Grace A McCarthy; Teena Nerwal; Avinoam Nevler; James B DuHadaway; Matthew D McCoy; Wei Jiang; Samantha Z Brown; Austin Goetz; Aditi Jain; Valerie S Calvert; Vikalp Vishwakarma; Dezhen Wang; Ranjan Preet; Joel Cassel; Ross Summer; Hoora Shaghaghi; Yves Pommier; Simone A Baechler; Michael J Pishvaian; Talia Golan; Charles J Yeo; Emanuel F Petricoin; George C Prendergast; Joseph Salvino; Pankaj K Singh; Dan A Dixon; Jonathan R Brody
Journal:  Mol Cancer Ther       Date:  2021-08-19       Impact factor: 6.261

2.  Pyrvinium targets autophagy addiction to promote cancer cell death.

Authors:  Longfei Deng; Yunlong Lei; Rui Liu; Jingyi Li; Kefei Yuan; Yi Li; Yi Chen; Yi Liu; You Lu; Carl K Edwards; Canhua Huang; Yuquan Wei
Journal:  Cell Death Dis       Date:  2013-05-02       Impact factor: 8.469

3.  Pyrvinium pamoate changes alternative splicing of the serotonin receptor 2C by influencing its RNA structure.

Authors:  Manli Shen; Stanislav Bellaousov; Michael Hiller; Pierre de La Grange; Trevor P Creamer; Orit Malina; Ruth Sperling; David H Mathews; Peter Stoilov; Stefan Stamm
Journal:  Nucleic Acids Res       Date:  2013-02-07       Impact factor: 16.971

4.  Deciphering the Role of Pyrvinium Pamoate in the Generation of Integrated Stress Response and Modulation of Mitochondrial Function in Myeloid Leukemia Cells through Transcriptome Analysis.

Authors:  Yu-Hsuan Fu; Chi-Yang Tseng; Jeng-Wei Lu; Wen-Hui Lu; Pei-Qi Lan; Chien-Yuan Chen; Da-Liang Ou; Liang-In Lin
Journal:  Biomedicines       Date:  2021-12-09
  4 in total

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