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Literature DB >> 17909890

Expression, purification, and characterization of a [Fe2S2] cluster containing ferredoxin from Acidithiobacillus ferrooxidans.

Jia Zeng¹, Xia Huang, Yuandong Liu, Jianshe Liu, Guanzhou Qiu.

Abstract

The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferredoxin from A. ferrooxidans ATCC 23270 was cloned, successfully expressed in Escherichia coli, and purified by one-step affinity chromatography to homogeneity. The MALDI-TOF MS and spectra results of the recombinant protein confirmed that the iron-sulfur cluster was correctly inserted into the active site of the protein. Site-directed mutagenesis results revealed that Cys42, Cys48, Cys51, and Cys87 were ligating with the [Fe(2)S(2)] cluster of the protein.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2007 PMID： 17909890 DOI： 10.1007/s00284-007-9025-4

Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188

18 in total

Review 1. Maturation of cellular Fe-S proteins: an essential function of mitochondria.

Authors: R Lill; G Kispal
Journal: Trends Biochem Sci Date: 2000-08 Impact factor: 13.807

2. Q-band ENDOR spectra of the Rieske protein from Rhodobactor capsulatus ubiquinol-cytochrome c oxidoreductase show two histidines coordinated to the [2Fe-2S] cluster.

Authors: R J Gurbiel; T Ohnishi; D E Robertson; F Daldal; B M Hoffman
Journal: Biochemistry Date: 1991-12-10 Impact factor: 3.162

3. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.

Authors: Y Takahashi; M Nakamura
Journal: J Biochem Date: 1999-11 Impact factor: 3.387

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters.

Authors: Y Kakuta; T Horio; Y Takahashi; K Fukuyama
Journal: Biochemistry Date: 2001-09-18 Impact factor: 3.162

6. Human ferredoxin: overproduction in Escherichia coli, reconstitution in vitro, and spectroscopic studies of iron-sulfur cluster ligand cysteine-to-serine mutants.

Authors: B Xia; H Cheng; V Bandarian; G H Reed; J L Markley
Journal: Biochemistry Date: 1996-07-23 Impact factor: 3.162

1. Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles.

Authors: Dan Zhou; Tangjian Peng; Hongbo Zhou; Xueduan Liu; Guohua Gu; Miao Chen; Guanzhou Qiu; Weimin Zeng
Journal: Curr Microbiol Date: 2015-05-05 Impact factor: 2.188

1 in total

Expression, purification, and characterization of a [Fe2S2] cluster containing ferredoxin from Acidithiobacillus ferrooxidans.

Review 1. Maturation of cellular Fe-S proteins: an essential function of mitochondria.

2. Q-band ENDOR spectra of the Rieske protein from Rhodobactor capsulatus ubiquinol-cytochrome c oxidoreductase show two histidines coordinated to the [2Fe-2S] cluster.

3. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters.

6. Human ferredoxin: overproduction in Escherichia coli, reconstitution in vitro, and spectroscopic studies of iron-sulfur cluster ligand cysteine-to-serine mutants.

Review 7. Recent developments in the field of iron-sulfur proteins.

8. Crystal structures of aconitase with isocitrate and nitroisocitrate bound.

Review 9. Structure-function studies of [2Fe-2S] ferredoxins.

Review 10. Aconitase, a two-faced protein: enzyme and iron regulatory factor.

1. Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles.