Literature DB >> 17907577

Semi-automated selection of DNA aptamers using magnetic particle handling.

Aniela Wochner1, Birgit Cech, Marcus Menger, Volker A Erdmann, Jörn Glökler.   

Abstract

We have developed a semi-automatic selection procedure for DNA aptamers. Employing a robotic workstation for magnetic particle handling, this method allows for a fast, reproducible, and parallelized selection of DNA aptamers. The selection protocol is designed to provide high flexibility and versatility in terms of choice of buffers and reagents, as well as stringency of selection. Using this procedure, we have successfully isolated ligand-specific, high-affinity DNA aptamers.

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Year:  2007        PMID： 17907577     DOI： 10.2144/000112532

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  13 in total

1.  Inhibition of Human Urokinase-Type Plasminogen Activator (uPA) Enzyme Activity and Receptor Binding by DNA Aptamers as Potential Therapeutics through Binding to the Different Forms of uPA.

Authors:  Nico Dreymann; Julia Wuensche; Wiebke Sabrowski; Anja Moeller; Denise Czepluch; Dana Vu Van; Susanne Fuessel; Marcus M Menger
Journal:  Int J Mol Sci       Date:  2022-04-28       Impact factor: 6.208

2.  Locked nucleic acid and aptamers.

Authors:  Kasper K Karlsen; Jesper Wengel
Journal:  Nucleic Acid Ther       Date:  2012-12       Impact factor: 5.486

3.  Probing the SELEX process with next-generation sequencing.

Authors:  Tatjana Schütze; Barbara Wilhelm; Nicole Greiner; Hannsjörg Braun; Franziska Peter; Mario Mörl; Volker A Erdmann; Hans Lehrach; Zoltán Konthur; Marcus Menger; Peter F Arndt; Jörn Glökler
Journal:  PLoS One       Date:  2011-12-29       Impact factor: 3.240

4.  Aptamers for pharmaceuticals and their application in environmental analytics.

Authors:  Beate Strehlitz; Christine Reinemann; Soeren Linkorn; Regina Stoltenburg
Journal:  Bioanal Rev       Date:  2011-12-17

Review 5.  MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing.

Authors:  Marcus Menger; Aysu Yarman; Júlia Erdőssy; Huseyin Bekir Yildiz; Róbert E Gyurcsányi; Frieder W Scheller
Journal:  Biosensors (Basel)       Date:  2016-07-18

Review 6.  Automation in the high-throughput selection of random combinatorial libraries--different approaches for select applications.

Authors:  Jörn Glökler; Tatjana Schütze; Zoltán Konthur
Journal:  Molecules       Date:  2010-04-08       Impact factor: 4.411

7.  A calibrated diversity assay for nucleic acid libraries using DiStRO--a Diversity Standard of Random Oligonucleotides.

Authors:  Tatjana Schütze; Peter F Arndt; Marcus Menger; Aniela Wochner; Martin Vingron; Volker A Erdmann; Hans Lehrach; Christian Kaps; Jörn Glökler
Journal:  Nucleic Acids Res       Date:  2009-12-03       Impact factor: 16.971

8.  Challenges and opportunities for small molecule aptamer development.

Authors:  Maureen McKeague; Maria C Derosa
Journal:  J Nucleic Acids       Date:  2012-10-24

9.  Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples.

Authors:  Corinna Wallinger; Karin Staudacher; Daniela Sint; Bettina Thalinger; Johannes Oehm; Anita Juen; Michael Traugott
Journal:  Ecol Evol       Date:  2017-07-07       Impact factor: 2.912

Review 10.  Predicting the Uncertain Future of Aptamer-Based Diagnostics and Therapeutics.

Authors:  John G Bruno
Journal:  Molecules       Date:  2015-04-16       Impact factor: 4.411
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