Germán Campuzano-Maya1. 1. School of Medicine, University of Antioquia, Medellín, Colombia. gcampuzano@hematologico.com
Abstract
AIM: To validate an optimized (13)C-urea breath test ((13)C-UBT) protocol for the diagnosis of H pylori infection that is cost-efficient and maintains excellent diagnostic accuracy. METHODS: 70 healthy volunteers were tested with two simplified (13)C-UBT protocols, with test meal (Protocol 2) and without test meal (Protocol 1). Breath samples were collected at 10, 20 and 30 min after ingestion of 50 mg (13)C-urea dissolved in 10 mL of water, taken as a single swallow, followed by 200 mL of water (pH 6.0) and a circular motion around the waistline to homogenize the urea solution. Performance of both protocols was analyzed at various cut-off values. Results were validated against the European protocol. RESULTS: According to the reference protocol, 65.7% individuals were positive for H pylori infection and 34.3% were negative. There were no significant differences in the ability of both protocols to correctly identify positive and negative H pylori individuals. However, only Protocol 1 with no test meal achieved accuracy, sensitivity, specificity, positive and negative predictive values of 100%. The highest values achieved by Protocol 2 were 98.57%, 97.83%, 100%, 100% and 100%, respectively. CONCLUSION: A 10 min, 50 mg (13)C-UBT with no test meal using a cut-off value of 2-2.5 is a highly accurate test for the diagnosis of H pylori infection at a reduced cost.
AIM: To validate an optimized (13)C-urea breath test ((13)C-UBT) protocol for the diagnosis of H pylori infection that is cost-efficient and maintains excellent diagnostic accuracy. METHODS: 70 healthy volunteers were tested with two simplified (13)C-UBT protocols, with test meal (Protocol 2) and without test meal (Protocol 1). Breath samples were collected at 10, 20 and 30 min after ingestion of 50 mg (13)C-urea dissolved in 10 mL of water, taken as a single swallow, followed by 200 mL of water (pH 6.0) and a circular motion around the waistline to homogenize the urea solution. Performance of both protocols was analyzed at various cut-off values. Results were validated against the European protocol. RESULTS: According to the reference protocol, 65.7% individuals were positive for H pylori infection and 34.3% were negative. There were no significant differences in the ability of both protocols to correctly identify positive and negative H pylori individuals. However, only Protocol 1 with no test meal achieved accuracy, sensitivity, specificity, positive and negative predictive values of 100%. The highest values achieved by Protocol 2 were 98.57%, 97.83%, 100%, 100% and 100%, respectively. CONCLUSION: A 10 min, 50 mg (13)C-UBT with no test meal using a cut-off value of 2-2.5 is a highly accurate test for the diagnosis of H pylori infection at a reduced cost.
Authors: P Malfertheiner; F Megraud; C O'Morain; F Bazzoli; E El-Omar; D Graham; R Hunt; T Rokkas; N Vakil; E J Kuipers Journal: Gut Date: 2006-12-14 Impact factor: 23.059
Authors: R L Riepl; C Folwaczny; B Otto; A Klauser; C Blendinger; B Wiebecke; A König; P Lehnert; W Heldwein Journal: Z Gastroenterol Date: 2000-01 Impact factor: 2.000
Authors: L G Coelho; M Reber; M C Passos; R O Aguiar; P E Casaes; M L Bueno; F R Yazaki; F J Castro; W L Vieira; J M Franco; L P Castro Journal: Braz J Med Biol Res Date: 1999-12 Impact factor: 2.590
Authors: Luigi Gatta; Nimish Vakil; Chiara Ricci; John F Osborn; Andrea Tampieri; Federico Perna; Mario Miglioli; Dino Vaira Journal: Am J Gastroenterol Date: 2004-05 Impact factor: 10.864
Authors: Fariborz Mansour-Ghanaei; Farahnaz Joukar; Mohammad Reza Sheykhian; Fatemeh Soati; Ali Mohammad Rafatzand Journal: Int J Clin Exp Med Date: 2013-03-21