OBJECTIVE: To determine the reliability of the baseline (BBAB) and post occlusion (POBAD) brachial artery diameters, brachial artery flow-mediated dilatations (BAFMD) measurements, and to quantify the typical error of the measurements (TEM). METHODS: Internal consistency (2 measurements on the same day) was determined in 10 volunteers, whereas stability (2 measurements on separate days) was determined in 13 volunteers. All the volunteers looked healthy and were nonsmokers. The brachial artery images were obtained using a two dimensional Doppler ultrasound instrument: a 14 MHz transducer was used. The distances between the intima-lumen interfaces were measured before and after the blood flow had been stopped by means of a cuff on the arm for five minutes. BAFMD was considered as the percentile increase of POBAD in relation to BBAD. RESULTS: ANOVA did not identify any significant differences between the measurements taken on the same and separate days. For BAFMD, the intraclass correlation coefficients between the measurements taken on the same and separate days were: R = 0.7001 and R = 0.8420, respectively (p < 0.05). The variation coefficients were 5.8% and 12.4% and the relative SMEs were 13.8% and 14.9%, respectively, for the measurements taken on the same and separate days. Analysis of the Bland-Altman graphs indicated that the variables did not present heteroscedastic errors. CONCLUSION: The BBAD, POBAD and BAFMD measurements using the manual ultrasound technique were highly reliable for both the same and separate day measurements and therefore can be used to diagnose and monitor endothelial function.
OBJECTIVE: To determine the reliability of the baseline (BBAB) and post occlusion (POBAD) brachial artery diameters, brachial artery flow-mediated dilatations (BAFMD) measurements, and to quantify the typical error of the measurements (TEM). METHODS: Internal consistency (2 measurements on the same day) was determined in 10 volunteers, whereas stability (2 measurements on separate days) was determined in 13 volunteers. All the volunteers looked healthy and were nonsmokers. The brachial artery images were obtained using a two dimensional Doppler ultrasound instrument: a 14 MHz transducer was used. The distances between the intima-lumen interfaces were measured before and after the blood flow had been stopped by means of a cuff on the arm for five minutes. BAFMD was considered as the percentile increase of POBAD in relation to BBAD. RESULTS: ANOVA did not identify any significant differences between the measurements taken on the same and separate days. For BAFMD, the intraclass correlation coefficients between the measurements taken on the same and separate days were: R = 0.7001 and R = 0.8420, respectively (p < 0.05). The variation coefficients were 5.8% and 12.4% and the relative SMEs were 13.8% and 14.9%, respectively, for the measurements taken on the same and separate days. Analysis of the Bland-Altman graphs indicated that the variables did not present heteroscedastic errors. CONCLUSION: The BBAD, POBAD and BAFMD measurements using the manual ultrasound technique were highly reliable for both the same and separate day measurements and therefore can be used to diagnose and monitor endothelial function.
Authors: Michael B Saddekni; Kenneth G Saag; Tanja Dudenbostel; Suzanne Oparil; David A Calhoun; Sebastian E Sattui; Daniel I Feig; Paul Muntner; David T Redden; Phillip J Foster; Elizabeth J Rahn; Stephanie R Biggers; Peng Li; Angelo L Gaffo Journal: Contemp Clin Trials Date: 2016-08-30 Impact factor: 2.226
Authors: Maristela Magnavita Oliveira Garcia; Carolina Garcez Varela; Patricia Fontes Silva; Paulo Roberto Passos Lima; Paulo Meira Góes; Marilia Galeffi Rodrigues; Maria de Lourdes Lima Souza E Silva; Ana Marice Teixeira Ladeia; Armênio Costa Guimarães; Luis Claudio Lemos Correia Journal: Arq Bras Cardiol Date: 2016-04 Impact factor: 2.000
Authors: Walter S Calderón-Gerstein; Antonio López-Peña; Raúl Macha-Ramírez; Astrid Bruno-Huamán; Roxana Espejo-Ramos; Stephany Vílchez-Bravo; María Ramírez-Breña; Milagros Damián-Mucha; Adriana Matos-Mucha Journal: Vasc Health Risk Manag Date: 2017-11-21