AIM OF THE STUDY: The anti-osteoporotic effect of heated powder of Ostreae Testa (hPOT), a powder of oyster shell-Ostrea gigas Thumb (Ostrediae) was observed in vitro and in vivo. MATERIALS AND METHODS: The effects on proliferation and alkaline phosphatase activity of primary osteoblasts, bone nodule formation, pit formation of osteoclasts and osteoclastogenesis were observed in vitro, and to observe the in vivo efficacy hPOT was orally administered once a day for 28 days to bilateral ovariectomy-induced osteoporosis mice at 125, 250 and 500mg/kg (of body weight). RESULTS: Although hPOT did not influence the pit formation and the number of multinucleated osteoclast-like cells, osteoclastogenesis, it enhanced the proliferation of primary osteoblasts, differentiation (ALP activity) and bone nodule formation of osteoblast in vitro. The estrogen-deficient osteoporotic changes were dramatically prevented by hPOT treatment except for osteoclasts/bone perimeter. CONCLUSIONS: In conclusion, hPOT prevents OVX-induced osteoporosis through osteoblasts activation effects.
AIM OF THE STUDY: The anti-osteoporotic effect of heated powder of Ostreae Testa (hPOT), a powder of oyster shell-Ostrea gigas Thumb (Ostrediae) was observed in vitro and in vivo. MATERIALS AND METHODS: The effects on proliferation and alkaline phosphatase activity of primary osteoblasts, bone nodule formation, pit formation of osteoclasts and osteoclastogenesis were observed in vitro, and to observe the in vivo efficacy hPOT was orally administered once a day for 28 days to bilateral ovariectomy-induced osteoporosismice at 125, 250 and 500mg/kg (of body weight). RESULTS: Although hPOT did not influence the pit formation and the number of multinucleated osteoclast-like cells, osteoclastogenesis, it enhanced the proliferation of primary osteoblasts, differentiation (ALP activity) and bone nodule formation of osteoblast in vitro. The estrogen-deficient osteoporotic changes were dramatically prevented by hPOT treatment except for osteoclasts/bone perimeter. CONCLUSIONS: In conclusion, hPOT prevents OVX-induced osteoporosis through osteoblasts activation effects.
Authors: José A Fernández Robledo; Raghavendra Yadavalli; Bassem Allam; Emmanuelle Pales Espinosa; Marco Gerdol; Samuele Greco; Rebecca J Stevick; Marta Gómez-Chiarri; Ying Zhang; Cynthia A Heil; Adrienne N Tracy; David Bishop-Bailey; Michael J Metzger Journal: Dev Comp Immunol Date: 2018-11-29 Impact factor: 3.636
Authors: Su Jin Kang; Beom Rak Choi; Seung Hee Kim; Hae Yeon Yi; Hye Rim Park; Chang Hyun Song; Sae Kwang Ku; Young Joon Lee Journal: Exp Ther Med Date: 2017-02-08 Impact factor: 2.447
Authors: Jae-Suk Choi; Joo Wan Kim; Ki Young Kim; Hyung-Rae Cho; In Soon Choi; Sae Kwang Ku Journal: Exp Ther Med Date: 2014-06-20 Impact factor: 2.447
Authors: Su Jin Kang; Beom Rak Choi; Seung Hee Kim; Hae Yeon Yi; Hye Rim Park; Chang Hyun Song; Sae Kwang Ku; Young Joon Lee Journal: Nutrients Date: 2016-07-23 Impact factor: 5.717