OBJECTIVE: The objective of the study was to determine alterations in cGMP, soluble guanylate cyclase (sGC), phosphodiesterase type 5 (PDE5), and B-type natriuretic peptide (BNP), in an animal model of a congenital cardiac defect with increased pulmonary blood flow. DESIGN: Prospective, comparative, experimental study. SUBJECTS: Lambs, from birth until 8 weeks of age. METHODOLOGY: Late gestation fetal lambs underwent in utero placement of an 8 mm aortopulmonary vascular graft (shunt). In shunted and normal age-matched control lambs, at 2, 4, and 8 weeks of age, cGMP and BNP levels were measured, and sGC subunit and PDE5 protein expression were determined by Western blot analysis and immunohistochemistry. RESULTS: In shunted lambs, tissue and plasma cGMP levels were greater than normal throughout the 8-week study period (P < 0.05). sGCalpha protein was greater at 2 and 4 weeks (P < 0.05), and sGCbeta and PDE5 protein were greater at 4 weeks in shunted lambs (P < 0.05). Plasma BNP levels did not change in normal lambs but increased in shunted lambs by 8 weeks of age (P < 0.05). BNP levels were greater in shunted lambs than normal at 4 and 8 weeks (P < 0.05). CONCLUSIONS: Alterations in sGC subunit protein expression during the first post-natal month, and increased BNP levels during the second post-natal month contribute to elevations in plasma and lung tissue cGMP in lambs with increased pulmonary blood flow. (c) 2007 Wiley-Liss, Inc.
OBJECTIVE: The objective of the study was to determine alterations in cGMP, soluble guanylate cyclase (sGC), phosphodiesterase type 5 (PDE5), and B-type natriuretic peptide (BNP), in an animal model of a congenital cardiac defect with increased pulmonary blood flow. DESIGN: Prospective, comparative, experimental study. SUBJECTS:Lambs, from birth until 8 weeks of age. METHODOLOGY: Late gestation fetal lambs underwent in utero placement of an 8 mm aortopulmonary vascular graft (shunt). In shunted and normal age-matched control lambs, at 2, 4, and 8 weeks of age, cGMP and BNP levels were measured, and sGC subunit and PDE5 protein expression were determined by Western blot analysis and immunohistochemistry. RESULTS: In shunted lambs, tissue and plasma cGMP levels were greater than normal throughout the 8-week study period (P < 0.05). sGCalpha protein was greater at 2 and 4 weeks (P < 0.05), and sGCbeta and PDE5 protein were greater at 4 weeks in shunted lambs (P < 0.05). Plasma BNP levels did not change in normal lambs but increased in shunted lambs by 8 weeks of age (P < 0.05). BNP levels were greater in shunted lambs than normal at 4 and 8 weeks (P < 0.05). CONCLUSIONS: Alterations in sGC subunit protein expression during the first post-natal month, and increased BNP levels during the second post-natal month contribute to elevations in plasma and lung tissue cGMP in lambs with increased pulmonary blood flow. (c) 2007 Wiley-Liss, Inc.
Authors: Rebecca Johnson Kameny; Sanjeev A Datar; Jason B Boehme; Catherine Morris; Terry Zhu; Brian D Goudy; Eric G Johnson; Csaba Galambos; Gary W Raff; Xutong Sun; Ting Wang; Samuel R Chiacchia; Qing Lu; Stephen M Black; Emin Maltepe; Jeffrey R Fineman Journal: Am J Respir Cell Mol Biol Date: 2019-05 Impact factor: 6.914
Authors: Saurabh Aggarwal; Christine M Gross; Sanjiv Kumar; Sanjeev Datar; Peter Oishi; Gokhan Kalkan; Christian Schreiber; Sohrab Fratz; Jeffrey R Fineman; Stephen M Black Journal: J Cell Physiol Date: 2011-12 Impact factor: 6.384
Authors: Shruti Sharma; Jubilee Barton; Ruslan Rafikov; Saurabh Aggarwal; Hsuan-Chang Kuo; Peter E Oishi; Sanjeev A Datar; Jeffrey R Fineman; Stephen M Black Journal: Pulm Pharmacol Ther Date: 2012-12-17 Impact factor: 3.410