| Literature DB >> 17901335 |
M Thomas P Gilbert1, Lynn P Tomsho, Snjezana Rendulic, Michael Packard, Daniela I Drautz, Andrei Sher, Alexei Tikhonov, Love Dalén, Tatyana Kuznetsova, Pavel Kosintsev, Paula F Campos, Thomas Higham, Matthew J Collins, Andrew S Wilson, Fyodor Shidlovskiy, Bernard Buigues, Per G P Ericson, Mietje Germonpré, Anders Götherström, Paola Iacumin, Vladimir Nikolaev, Malgosia Nowak-Kemp, Eske Willerslev, James R Knight, Gerard P Irzyk, Clotilde S Perbost, Karin M Fredrikson, Timothy T Harkins, Sharon Sheridan, Webb Miller, Stephan C Schuster.
Abstract
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.Entities:
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Year: 2007 PMID： 17901335 DOI： 10.1126/science.1146971
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728