PURPOSE: The aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes. METHODS: The spindles of 134 in vivo matured, 105 in vitro matured oocytes were examined by Polscope at the time of ICSI. RESULTS: The spindles were visualized in 83.6 and 77.1% of in vivo and in vitro matured oocytes respectively. The rate of fertilization of in vivo matured oocytes with spindles beneath or adjacent to the first polar body (angle of 0-5 degrees) was significantly higher (93.3%) than all other groups. The proportions of various spindle positions did not differ statistically in in vivo and in vitro matured oocytes. CONCLUSIONS: Meiotic spindle location with regard to the first polar body appears to influence fertilization rate.
PURPOSE: The aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes. METHODS: The spindles of 134 in vivo matured, 105 in vitro matured oocytes were examined by Polscope at the time of ICSI. RESULTS: The spindles were visualized in 83.6 and 77.1% of in vivo and in vitro matured oocytes respectively. The rate of fertilization of in vivo matured oocytes with spindles beneath or adjacent to the first polar body (angle of 0-5 degrees) was significantly higher (93.3%) than all other groups. The proportions of various spindle positions did not differ statistically in in vivo and in vitro matured oocytes. CONCLUSIONS: Meiotic spindle location with regard to the first polar body appears to influence fertilization rate.
Authors: Alina M Mahfoudh; Jeong H Moon; Sara Henderson; Elena Garcia-Cerrudo; Weon-Young Son; Michael H Dahan Journal: J Assist Reprod Genet Date: 2017-02-28 Impact factor: 3.412
Authors: Luciana A Dib; Maria C P M Araújo; Roberta Cristina Giorgenon; Gustavo S Romão; Rui A Ferriani; Paula A Navarro Journal: Reprod Sci Date: 2012-09-18 Impact factor: 3.060